Vela Ramirez Julia E, Boggiatto Paola M, Wannemuehler Michael J, Narasimhan Balaji
Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, United States.
Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011, United States.
ACS Biomater Sci Eng. 2017 Feb 13;3(2):160-168. doi: 10.1021/acsbiomaterials.6b00394. Epub 2016 Oct 6.
An in-depth understanding of the interactions of vaccine delivery vehicles with antigen presenting cells is important for tailoring optimal adjuvant properties. Polymeric nanoparticles have been widely studied as adjuvants and delivery vehicles; however, there is little information regarding the effect of serum protein adsorption onto biomaterials and the effect of this adsorption upon interactions with antigen presenting cells. The current studies analyzed effects of polyanhydride chemistry on serum adsorption to nanoparticles with respect to their uptake by and activation of bone marrow-derived macrophages. Differential effects of serum adsorption based on nanoparticle chemistry were shown to enhance (for 1,6-bis(-carboxyphenoxy)hexane and sebacic anhydride-based) or reduce (for 1,6-bis(-carboxyphenoxy)hexane and 1,8-bis(-carboxyphenoxy)-3,6-dioxaoctane-based) nanoparticle uptake. The observed complex interdependence between nanoparticle chemistry and serum protein adsorption on macrophage activation provided insights that will facilitate the rational design of single-dose nanovaccines developed to induce robust immune responses.
深入了解疫苗递送载体与抗原呈递细胞之间的相互作用对于定制最佳佐剂特性至关重要。聚合物纳米颗粒作为佐剂和递送载体已被广泛研究；然而，关于血清蛋白吸附到生物材料上的影响以及这种吸附对与抗原呈递细胞相互作用的影响，目前几乎没有相关信息。当前的研究分析了聚酐化学对纳米颗粒血清吸附的影响，以及其对骨髓来源巨噬细胞摄取和激活的影响。结果表明，基于纳米颗粒化学的血清吸附差异效应会增强（对于基于1,6 - 双（- 羧基苯氧基）己烷和癸二酸酐的纳米颗粒）或降低（对于基于1,6 - 双（- 羧基苯氧基）己烷和1,8 - 双（- 羧基苯氧基）- 3,6 - 二氧杂辛烷的纳米颗粒）纳米颗粒的摄取。观察到的纳米颗粒化学与血清蛋白吸附对巨噬细胞激活之间复杂的相互依存关系提供了相关见解，这将有助于合理设计用于诱导强烈免疫反应的单剂量纳米疫苗。
