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自聚集胶原图案控制细胞行为。

Self-agglomerated collagen patterns govern cell behaviour.

机构信息

Biointerface Science Group, Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands.

Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands.

出版信息

Sci Rep. 2021 Jan 15;11(1):1516. doi: 10.1038/s41598-021-81054-5.

Abstract

Reciprocity between cells and their surrounding extracellular matrix is one of the main drivers for cellular function and, in turn, matrix maintenance and remodelling. Unravelling how cells respond to their environment is key in understanding mechanisms of health and disease. In all these examples, matrix anisotropy is an important element, since it can alter the cell shape and fate. In this work, the objective is to develop and exploit easy-to-produce platforms that can be used to study the cellular response to natural proteins assembled into diverse topographical cues. We demonstrate a robust and simple approach to form collagen substrates with different topographies by evaporating droplets of a collagen solution. Upon evaporation of the collagen solution, a stain of collagen is left behind, composed of three regions with a distinct pattern: an isotropic region, a concentric ring pattern, and a radially oriented region. The formation and size of these regions can be controlled by the evaporation rate of the droplet and initial collagen concentration. The patterns form topographical cues inducing a pattern-specific cell (tenocyte) morphology, density, and proliferation. Rapid and cost-effective production of different self-agglomerated collagen topographies and their interfaces enables further study of the cell shape-phenotype relationship in vitro. Substrate topography and in analogy tissue architecture remains a cue that can and will be used to steer and understand cell function in vitro, which in turn can be applied in vivo, e.g. in optimizing tissue engineering applications.

摘要

细胞与其周围细胞外基质之间的相互作用是细胞功能的主要驱动力之一,进而也是基质维持和重塑的主要驱动力。揭示细胞如何对其环境做出反应是理解健康和疾病机制的关键。在所有这些例子中,基质各向异性是一个重要的因素,因为它可以改变细胞的形状和命运。在这项工作中,目的是开发和利用易于生产的平台,用于研究细胞对组装成各种形貌特征的天然蛋白质的反应。我们展示了一种通过蒸发胶原溶液滴来制备具有不同形貌的胶原底物的稳健而简单的方法。在胶原溶液蒸发后,会留下一层胶原染料,由三个具有不同图案的区域组成:各向同性区域、同心环图案区域和放射状取向区域。这些区域的形成和大小可以通过液滴的蒸发速率和初始胶原浓度来控制。这些图案形成了形貌特征,诱导特定细胞(肌腱细胞)形态、密度和增殖。快速且具有成本效益的生产不同自聚集胶原形貌及其界面,使我们能够进一步研究细胞体外形态-表型关系。与组织架构类似,基底形貌仍然是一个可以用来引导和理解细胞体外功能的线索,这反过来又可以应用于体内,例如优化组织工程应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8839/7810981/c8bdfc162d45/41598_2021_81054_Fig1_HTML.jpg

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