可控表面形貌和化学性质对小鼠胚胎干细胞黏附、生长及自我更新的作用
The Role of Controlled Surface Topography and Chemistry on Mouse Embryonic Stem Cell Attachment, Growth and Self-Renewal.
作者信息
Macgregor Melanie, Williams Rachel, Downes Joni, Bachhuka Akash, Vasilev Krasimir
机构信息
School of Engineering, Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.
Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool L7 8TX, UK.
出版信息
Materials (Basel). 2017 Sep 14;10(9):1081. doi: 10.3390/ma10091081.
The success of stem cell therapies relies heavily on our ability to control their fate in vitro during expansion to ensure an appropriate supply. The biophysical properties of the cell culture environment have been recognised as a potent stimuli influencing cellular behaviour. In this work we used advanced plasma-based techniques to generate model culture substrates with controlled nanotopographical features of 16 nm, 38 nm and 68 nm in magnitude, and three differently tailored surface chemical functionalities. The effect of these two surface properties on the adhesion, spreading, and self-renewal of mouse embryonic stem cells (mESCs) were assessed. The results demonstrated that physical and chemical cues influenced the behaviour of these stem cells in in vitro culture in different ways. The size of the nanotopographical features impacted on the cell adhesion, spreading and proliferation, while the chemistry influenced the cell self-renewal and differentiation.
干细胞疗法的成功在很大程度上依赖于我们在体外扩增过程中控制其命运以确保适当供应的能力。细胞培养环境的生物物理特性已被认为是影响细胞行为的有力刺激因素。在这项工作中，我们使用先进的基于等离子体的技术来生成具有16纳米、38纳米和68纳米量级可控纳米拓扑特征以及三种不同定制表面化学功能的模型培养底物。评估了这两种表面特性对小鼠胚胎干细胞（mESCs）的黏附、铺展和自我更新的影响。结果表明，物理和化学线索以不同方式影响这些干细胞在体外培养中的行为。纳米拓扑特征的大小影响细胞的黏附、铺展和增殖，而化学性质则影响细胞的自我更新和分化。
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