Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic.
Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague 4, Czech Republic.
Sci Rep. 2021 Jan 15;11(1):1541. doi: 10.1038/s41598-021-81083-0.
There remains to this day a great gap in understanding as to the role of B cells and their products-antibodies and cytokines-in mediating the protective response to Francisella tularensis, a Gram-negative coccobacillus belonging to the group of facultative intracellular bacterial pathogens. We previously have demonstrated that Francisella interacts directly with peritoneal B-1a cells. Here, we demonstrate that, as early as 12 h postinfection, germ-free mice infected with Francisella tularensis produce infection-induced antibody clones reacting with Francisella tularensis proteins having orthologs or analogs in eukaryotic cells. Production of some individual clones was limited in time and was influenced by virulence of the Francisella strain used. The phylogenetically stabilized defense mechanism can utilize these early infection-induced antibodies both to recognize components of the invading pathogens and to eliminate molecular residues of infection-damaged self cells.
时至今日，人们对于 B 细胞及其产物（抗体和细胞因子）在介导对土拉弗朗西斯菌（一种革兰氏阴性短杆菌，属于兼性细胞内细菌病原体群）的保护性反应中的作用仍存在很大的理解差距。我们之前已经证明，弗朗西斯菌直接与腹膜 B-1a 细胞相互作用。在这里，我们证明，早在感染后 12 小时，感染土拉弗朗西斯菌的无菌小鼠就会产生感染诱导的抗体克隆，这些克隆与真核细胞中具有同源物或类似物的土拉弗朗西斯菌蛋白反应。一些个体克隆的产生是时间有限的，并受到所用弗朗西斯菌菌株毒力的影响。这种进化上稳定的防御机制可以利用这些早期感染诱导的抗体来识别入侵病原体的成分，并消除感染损伤自身细胞的分子残留。
