Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Department of Pathology, School of Medicine, Keio University, Tokyo, 160-8582, Japan.
Sci Rep. 2021 Jan 15;11(1):1515. doi: 10.1038/s41598-021-81027-8.
Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hypertrophy was significantly more prominent in diabetic mice than in nondiabetic mice, and this was evident even at the early stage. Additionally, the number of vacuoles in PECs was markedly increased in diabetic mice, suggesting the presence of cellular injury in PECs in DN. Although rare, binuclear cells were observed in mice with early diabetes. In cultured PECs, a high glucose condition, compared with normal glucose condition, induced cellular hypertrophy and apoptosis. Flow cytometry showed that some PECs in the G0 phase reentered the cell cycle but got arrested in the S phase. Finally, in human diabetic subjects, hypertrophy and vacuolization were observed in the PECs. Our data showed that PECs undergo substantial changes in DN and may participate in rearrangement for differentiation into podocytes.
糖尿病肾病 (DN) 伴有肾小球的特征性改变，但对于糖尿病对壁细胞 (PEC) 的影响知之甚少。在这项研究中，对糖尿病 db/db 小鼠和糖尿病患者的 PEC 进行了描述性分析。与非糖尿病小鼠相比，糖尿病小鼠的 PEC 肥大更为明显，即使在早期也是如此。此外，糖尿病小鼠 PEC 中的空泡数量明显增加，提示 DN 中 PEC 存在细胞损伤。尽管很少见，但在早期糖尿病小鼠中观察到双核细胞。在培养的 PEC 中，与正常葡萄糖条件相比，高葡萄糖条件诱导细胞肥大和凋亡。流式细胞术显示，G0 期的一些 PEC 重新进入细胞周期，但在 S 期停滞。最后，在人类糖尿病患者中，观察到 PEC 的肥大和空泡化。我们的数据表明，DN 中 PEC 发生了实质性变化，可能参与了向足细胞分化的重排。
