人胎盘刷状缘膜囊泡对硒（硒酸盐）的转运

Selenium (selenate) transport by human placental brush border membrane vesicles.

Shennan D B

Department of Human Anatomy, University of Oxford.

Br J Nutr. 1988 Jan;59(1):13-9. doi: 10.1079/bjn19880005.

Abstract

Selenate uptake by human placental brush-border-membrane vesicles was studied in order to establish whether this anion shares a pathway with sulphate. 2. Selenate uptake was found to be saturable with respect to medium selenate and was inhibited by the anion exchange inhibitor 4,4'-diisothiocyano-stilbene-2,2'-disulphonate (DIDS). 3. Anions which have a similar tetrahedral shape to selenate, e.g. chromate, molybdate, tungstate and sulphate, were effective inhibitors of selenate uptake when added to the incubation medium. 4. Sulphate inhibited selenate influx in a dose-dependent fashion; moreover sulphate was found to be a competitive inhibitor of selenate uptake. 5. It is concluded that selenate and sulphate share a pathway for transport in the human placental microvillus membrane.

摘要

为确定这种阴离子是否与硫酸盐共用一条转运途径，对人胎盘刷状缘膜囊泡摄取硒酸盐的情况进行了研究。2. 发现硒酸盐摄取相对于培养基中的硒酸盐具有饱和性，并受到阴离子交换抑制剂4,4'-二异硫氰酸根合芪-2,2'-二磺酸盐（DIDS）的抑制。3. 当添加到孵育培养基中时，与硒酸盐具有相似四面体形状的阴离子，如铬酸盐、钼酸盐、钨酸盐和硫酸盐，是硒酸盐摄取的有效抑制剂。4. 硫酸盐以剂量依赖的方式抑制硒酸盐内流；此外，发现硫酸盐是硒酸盐摄取的竞争性抑制剂。5. 得出的结论是，硒酸盐和硫酸盐在人胎盘微绒毛膜中共享一条转运途径。