Division of Cardiovascular Medicine, Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Department of Dermatology, University of Wisconsin, Madison, WI, USA.
Exp Cell Res. 2021 Feb 15;399(2):112489. doi: 10.1016/j.yexcr.2021.112489. Epub 2021 Jan 14.
Cardiac fibroblasts and myofibroblasts assemble and maintain extracellular matrix during normal development and following injury. Culture expansion of these cells yield a bioengineered matrix that could lead to intriguing therapeutic opportunities. For example, we reported that cultured rat cardiac fibroblasts form a matrix that can be used to delivery therapeutic stem cells. Furthermore, we reported that matrix derived from cultured human cardiac fibroblasts/myofibroblasts converted monocytes into macrophages that express interesting anti-inflammatory and pro-angiogenic properties. Expanding these matrix investigations require characterization of the source cells for quality control. In these efforts, we observed and herein report that Sushi Containing Domain 2 (SUSD2) is a novel and consistent marker for cultured human cardiac fibroblast and myofibroblasts.
心脏成纤维细胞和肌成纤维细胞在正常发育和损伤后组装并维持细胞外基质。这些细胞的培养扩增产生了一种生物工程基质，可能会带来有趣的治疗机会。例如，我们报道了培养的大鼠心脏成纤维细胞形成的基质可用于递送治疗性干细胞。此外，我们还报道了源自培养的人心脏成纤维细胞/肌成纤维细胞的基质将单核细胞转化为表达有趣的抗炎和促血管生成特性的巨噬细胞。为了扩大这些基质的研究，需要对源细胞进行特征描述，以进行质量控制。在这些研究中，我们观察到并在此报告，富含 Sushi 结构域蛋白 2（SUSD2）是培养的人心脏成纤维细胞和肌成纤维细胞的一种新型且一致的标志物。
