The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China.
The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China; Department of Food and Environmental Sciences, University of Helsinki, Finland.
Int J Biol Macromol. 2021 Mar 15;173:399-408. doi: 10.1016/j.ijbiomac.2021.01.052. Epub 2021 Jan 14.
Herein, a new method was demonstrated for effective immobilization of the antibacterial peptide nisin on Grifola frondosa hydrophobin (HGFI), without the need of any additional complex reaction. Hydrophobin can self-assemble as a monolayer to form continuous negative-charged surfaces with enhanced wettability and biocompatibility. Adding nisin solution to such hydrophobin surface created antibacterial surfaces. The quantification analysis revealed that more nisin could be adsorbed on the HGFI-coated than to control polystyrene surfaces at different pH values. This suggested that electronic attraction and wettability may play important roles in this process. The transmission electron microscopy, atomic force microscopy and fourier transform infrared (FTIR) analysis indicated the adsorption mode of nisin on the HGFI film, i.e., hydrophobins served as an adhesive layer for binding charged peptides to interfaces. The antibacterial activity of the treated surface was investigated via counting, a nucleic acid release test, scanning electron microscopy, and biofilm detection. These results indicated the excellent antibacterial activity of nisin adsorbed on the HGFI-coated surfaces. The activity retention of adsorbed nisin was demonstrated by immersing the modified substrates in a flowed liquid condition.
本文展示了一种新方法，可有效将抗菌肽乳链菌肽固定在灰树花疏水性蛋白（HGFI）上，而无需任何其他复杂反应。疏水性蛋白可以自组装成单层，形成具有增强润湿性和生物相容性的连续带负电荷表面。将乳链菌肽溶液添加到这种疏水性蛋白表面会形成抗菌表面。定量分析表明，在不同 pH 值下，更多的乳链菌肽可以吸附在 HGFI 涂层上，而不是控制聚苯乙烯表面。这表明电子吸引力和润湿性可能在这个过程中发挥重要作用。透射电子显微镜、原子力显微镜和傅里叶变换红外（FTIR）分析表明了乳链菌肽在 HGFI 膜上的吸附模式，即疏水性蛋白作为带电荷肽与界面结合的粘合层。通过计数、核酸释放试验、扫描电子显微镜和生物膜检测研究了处理表面的抗菌活性。这些结果表明，吸附在 HGFI 涂层表面上的乳链菌肽具有优异的抗菌活性。通过将改性基底浸泡在流动液体条件下，证明了吸附乳链菌肽的活性保留。
