Uptake, accumulation, and translocation of Zn, Cu, Pb, Cd, Ni, and Cr by P. australis seedlings in an urban dredged sediment mesocosm: Impact of seedling origin and initial trace metal content.

The study presents results from 6 months of phytoremediation of sediments dredged from three urban retention tanks carried out in a mesocosm setup with the use of P. australis. Two kinds of P. australis seedlings were considered: seedlings originating from natural (uncontaminated - S) and anthropogenically changed environments (contaminated - S); this distinction was reflected in the baseline concentrations of trace metals inside their tissues. The potentially toxic elements (PTEs) considered in this study were as follows: Zn, Cu, Cd, Ni, Cr, and Pb. The aim of the study was to compare the uptake, accumulation, and translocation properties of seedlings with different initial trace metal contents. The PTE concentrations were analyzed in sediments as well as in belowground and aboveground parts of plants in the middle (3rd month) and at the end of the investigation period using inductively coupled plasma mass spectrometry (ICP-MS), and the accumulation of PTEs in plant tissues was calculated. Phytoextraction efficiency was evaluated using the bioconcentration factor (BF) and translocation factor (TF). Plant morphology was assessed with scanning electron microscopy (SEM) to document plant stress due to PTE exposure. The results of our study indicated that P. australis seedlings originating from sites differing in the initial trace metal content exhibited different behavior when grown on sediments dredged from urban retention tanks. S seedlings with low initial metal contents tended to adapt to the dredged sediments and showed phytoextraction ability, while S seedlings originating from sites with initial high contents of trace metals acted as phytoexcluders and tended to release PTEs from their tissues into the sediments. The morphological and structural effects caused by metal toxicity were observed in growth limitation, root tissue disturbance, root hair number decrease, and structural alterations in the epidermis and endodermis. Therefore, the S seedlings presented better properties and adaptability for phytoremediation purposes.