固定化蒽醌-2-磺酸钠对厌氧反应器生物膜中抗生素抗性基因和微生物群落的影响。
Effect of immobilized anthraquinone-2-sulfonate on antibiotic resistance genes and microbial community in biofilms of anaerobic reactors.
机构信息
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.
出版信息
J Environ Manage. 2021 Mar 15;282:111967. doi: 10.1016/j.jenvman.2021.111967. Epub 2021 Jan 14.
Quinone compounds could significantly accelerate anaerobic biotransformation of refractory pollutants. However, the effect of quinone compounds application on the propagation of antibiotic resistance genes (ARGs) in the bio-treatment of these pollutants-containing wastewater is not available. In this study, the catalytic performance of anthraquinone-2-sulfonate immobilized on polyurethane foam (AQS-PUF), changes of ARGs, mobile gene elements (MGEs) and microbial community structure attached on AQS-PUF and PUF in the up-flow anaerobic bioreactors were investigated. The results showed that AQS-PUF could significantly accelerate the decolorization of azo dye RR X-3B. Meanwhile, metagenomics analysis showed that the total absolute abundance of ARGs increased in the presence of the immobilized AQS. Among ARGs, the number of the efflux pump-encoding ARGs in the biofilm of AQS-PUF accounted for 35.7% of the total ARGs, which was slightly higher than that of PUF (32.1%) due to the presence of the immobilized AQS. The relative abundances of ARGs conferring resistance to MLS (macrolide, lincosamide and streptogramin), tetracycline and sulfonamide, which were deeply concerned, reduced 10%, 21.7% and 7.3% in the presence of the immobilized AQS, respectively. Moreover, the immobilized AQS resulted in the decreased relative abundance of plasmids, transposons and class I integrons. Among the detected 31 ARG subtypes located in MGEs, the relative abundances of only lnuF, msrE and mphD in the biofilm of AQS-PUF were over 2-fold higher compared with those in the biofilm of PUF. However, the three ARGs and their host Gammaproteobacteria was not dominant in microbial community. The relative abundances of more ARGs including MLS (lnuB and EreA), tetracycline (tetH) resistance genes located in MGEs decreased, which was attributed to the decreased relative abundance of their hosts. These studies showed that the addition of the immobilized AQS (around 0.25 mM) had a beneficial effect on reducing the spread of ARGs during dyeing wastewater bio-treatment.
醌类化合物可以显著加速难生物降解污染物的厌氧生物转化。然而，醌类化合物在这些含污染物废水的生物处理中对抗生素抗性基因（ARGs）的传播的影响尚不清楚。在这项研究中，研究了固定在聚氨酯泡沫上的蒽醌-2-磺酸盐（AQS-PUF）的催化性能、ARGs、移动基因元件（MGEs）以及在升流式厌氧生物反应器中附着在 AQS-PUF 和 PUF 上的微生物群落结构的变化。结果表明，AQS-PUF 可以显著加速偶氮染料 RR X-3B 的脱色。同时，宏基因组学分析表明，固定化 AQS 的存在增加了 ARGs 的总绝对丰度。在 ARGs 中，AQS-PUF 生物膜中编码外排泵的 ARGs 数量占总 ARGs 的 35.7%，略高于 PUF（32.1%），这是由于固定化 AQS 的存在。对 MLS（大环内酯类、林可酰胺类和链阳菌素类）、四环素和磺胺类抗生素具有抗性的 ARGs 的相对丰度分别降低了 10%、21.7%和 7.3%。此外，固定化 AQS 导致质粒、转座子和 I 类整合子的相对丰度降低。在所检测到的位于 MGEs 中的 31 种 ARG 亚型中，AQS-PUF 生物膜中 lnuF、msrE 和 mphD 的相对丰度均高于 PUF 生物膜中的 2 倍。然而，这三个 ARG 及其宿主γ变形菌在微生物群落中并不占优势。位于 MGEs 中的更多 ARG（包括 MLS（lnuB 和 EreA）、四环素（tetH）抗性基因）的相对丰度降低，这归因于其宿主的相对丰度降低。这些研究表明，在染色废水生物处理过程中，添加固定化 AQS（约 0.25 mM）对减少 ARGs 的传播有有益的影响。
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