The First Research Institute of the Ministry of Public Security P.R.C, Beijing, People's Republic of China.
Department of Forensic Science, School of Basic Medical Sciences, Central South University, No172. Tongzipo Road, Changsha, 410013, Hunan Province, People's Republic of China.
Int J Legal Med. 2021 Mar;135(2):431-440. doi: 10.1007/s00414-020-02490-y. Epub 2021 Jan 16.
Genotyping of short tandem repeat (STR) markers is the basic method of forensic science. Enhanced technologies are needed to meet the requirements of databasing and casework samples. The STRscan-17LC kit is a 6 Dye STR kit which amplifies 16 STR loci: D3S1358, TPOX, D16S539, vWA, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, D18S51, TH01, D12S391, and D21S11 and the sex-determinant locus amelogenin. This kit is designed for better tolerance to PCR inhibitors and analysis of mildly degraded samples with all fragments smaller than 330 bases. In this study, the STRscan-17LC kit is validated according to the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines, including PCR-based studies, sensitivity, precision and accuracy, inhibitors, species specificity, DNA mixture studies, population, and concordance studies. The validation results suggest that the STRscan-17LC kit is a useful tool for forensic application.
短串联重复序列（STR）基因分型是法医学的基本方法。需要增强技术来满足数据库和案例样本的要求。STRscan-17LC 试剂盒是一种 6 色荧光 STR 试剂盒，可扩增 16 个 STR 基因座：D3S1358、TPOX、D16S539、vWA、D2S1338、CSF1PO、D19S433、D7S820、FGA、D8S1179、D5S818、D13S317、D18S51、TH01、D12S391 和 D21S11 以及性别决定基因座 amelogenin。该试剂盒设计用于更好地耐受 PCR 抑制剂，并分析所有小于 330 个碱基的片段较小的轻度降解样本。在这项研究中，根据 SWGDAM（DNA 分析方法科学工作组）指南对 STRscan-17LC 试剂盒进行了验证，包括基于 PCR 的研究、灵敏度、精密度和准确性、抑制剂、种属特异性、DNA 混合研究、群体和一致性研究。验证结果表明，STRscan-17LC 试剂盒是法医学应用的有用工具。
