The First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Int J Legal Med. 2021 Nov;135(6):2263-2274. doi: 10.1007/s00414-021-02695-9. Epub 2021 Sep 22.
DNA profiling of short tandem repeats (STRs) is the primary method for genotyping forensic samples. However, degraded DNA and trace samples are still major problems for commercial 5- or 6-dye STR kits. In order to improve the performance of this method, we developed a novel 8-dye STR multiplex system containing 18 autosomal loci (D3S1358, D1S1656, TPOX, D16S539, vWA, D6S1043, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, TH01, D21S11, D12S391, and PentaD) and the sex-determining locus Amelogenin, with all fragments smaller than 330 bases. Validation was carried out as recommended by the Scientific Working Group on DNA Analysis Methods. The results showed that complete profiles were obtainable when the input DNA was as low as 0.0625 ng. Full profiles were obtained even in the presence of inhibitors such as humic acid (< 300 ng/μl), hematin (< 100 μM), and indigo (0.01%). The 8-dye STR multiplex system also showed good performance in the detection degraded DNA samples. These results indicate that the 8-dye STR multiplex system is suitable for human DNA genotyping, including for difficult forensic materials.
短串联重复序列（STR）的 DNA 图谱分析是基因分型法医样本的主要方法。然而，降解的 DNA 和微量样本仍然是商业 5 或 6 色 STR 试剂盒的主要问题。为了提高该方法的性能，我们开发了一种新的 8 色 STR 多重体系，包含 18 个常染色体基因座（D3S1358、D1S1656、TPOX、D16S539、vWA、D6S1043、D2S1338、CSF1PO、D19S433、D7S820、FGA、D8S1179、D5S818、D13S317、TH01、D21S11、D12S391 和 PentaD）和性别决定基因座 Amelogenin，所有片段均小于 330 个碱基。验证是按照 DNA 分析方法科学工作组的建议进行的。结果表明，当输入 DNA 低至 0.0625ng 时，即可获得完整的图谱。即使存在腐殖酸（<300ng/μl）、胆素（<100μM）和靛蓝（0.01%）等抑制剂，也能获得完整的图谱。8 色 STR 多重体系在检测降解 DNA 样本时也表现出良好的性能。这些结果表明，8 色 STR 多重体系适用于人类 DNA 基因分型，包括困难的法医材料。
