BK21 PLUS Center for Advanced Chemical Technology, Department of Polymer Science and Engineering, Pusan National University, Busan 609-735, Republic of Korea.
Department of Polymer Science and Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
ACS Biomater Sci Eng. 2020 Sep 14;6(9):5012-5023. doi: 10.1021/acsbiomaterials.0c00585. Epub 2020 Aug 25.
Multistimuli-responsive nanomedicines present great potential for cancer therapy, as they can be featured as simple, selective, and smart carriers that can release their payload on-demand. In this study, we prepared a multifunctional polymeric vesicular nanocarrier (PVN) based on robust and triple stimuli-responsive micelles that could encapsulate chemotherapeutic drugs (doxorubicin (DOX)) and photothermal agents (IR780 iodide) for combined chemo-photothermal therapy. The size of the PVNs was stable and uniform (∼100 nm), and its DOX and IR780 loading were high: 26.5 and 16.4 wt %, respectively. Further investigations suggested that the DOX/IR780 coloaded PVNs presented controlled drug release kinetics upon costimulation with specific endogenous stimuli. Upon laser irradiation, DOX/IR780 coloaded PVNs exhibited prominent photothermal cytotoxicity toward murine colon cancer (CT-26) cells. Intracellular uptake assays indicated that DOX/IR780 coloaded PVNs could be readily uptaken by CT-26 cells, resulting in the release of DOX within the cytoplasm of the cells in response to laser irradiation.
多功能聚合物囊泡纳米载体（PVN）的制备及其用于协同化疗-光热治疗
多刺激响应纳米药物在癌症治疗中具有很大的应用潜力，因为它们可以作为简单、选择性和智能载体，按需释放其负载物。在本研究中，我们制备了一种基于坚固的三重刺激响应胶束的多功能聚合物囊泡纳米载体（PVN），该胶束可以包载化疗药物（阿霉素（DOX））和光热剂（IR780 碘化物），用于联合化疗-光热治疗。PVN 的尺寸稳定且均匀（约 100nm），其 DOX 和 IR780 的载药量分别高达 26.5wt%和 16.4wt%。进一步的研究表明，DOX/IR780 共载 PVN 在受到特定内源性刺激的双重刺激时表现出可控的药物释放动力学。在激光照射下，DOX/IR780 共载 PVN 对小鼠结肠癌细胞（CT-26）表现出显著的光热细胞毒性。细胞内摄取实验表明，DOX/IR780 共载 PVN 可以被 CT-26 细胞轻易摄取，导致 DOX 在激光照射下在细胞质内释放。
