Division of Biomedical Engineering, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran 14395-1561, Iran.
School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, Tehran 14155, Iran.
ACS Biomater Sci Eng. 2020 Apr 13;6(4):2094-2106. doi: 10.1021/acsbiomaterials.0c00263. Epub 2020 Apr 2.
The addition of noble elements such as Ag was shown as a successful method to accelerate the corrosion rate of absorbable Fe-based alloys. One major concern of Ag addition is its effect on hemocompatibility and biocompatibility. In this study, in vitro degradation and surface analysis of Fe-30Mn-Ag ( = 0, 1, and 3 wt %) alloys as well as their effects on hemocompatibility and cell viability of human umbilical vein endothelial cells (HUVECs) were investigated. The static degradation rate of the alloys was 4.97, 4.69, and 4.49 mg/cm for Fe-30Mn, Fe-30Mn-1Ag, and Fe-30Mn-3Ag, respectively. The surface analysis after degradation showed that γ-FeOOH was formed on Fe-30Mn-3Ag, while α-FeOOH was more dominant on Fe-30Mn and Fe-30Mn-1Ag. As γ-FeOOH is more soluble than α-FeOOH, it assists further degradation of Fe-30Mn-3Ag alloy. The high amount of Ag, which induced the hemolysis ratio, however, inhibited coagulation by decreasing the platelet adhesion. Fe-30Mn-1Ag and Fe-30Mn-3Ag alloys show an improved cell viability as compared to that of Fe-Mn alloy. Shear yield strength and shear elastic modulus of the samples after immersion tests were increased, while the ultimate shear strength was not affected. On the basis of the acceptable hemolysis rate, low platelet adhesion, acceptable cell viability, and appropriate mechanical properties after degradation, Fe-30Mn-1Ag can be considered as a suitable blood-contacting Fe-based absorbable alloy.
添加银等贵金属元素被证明是加速可吸收铁基合金腐蚀速率的一种有效方法。银添加的一个主要关注点是其对血液相容性和生物相容性的影响。本研究研究了 Fe-30Mn-Ag（=0、1 和 3wt%）合金的体外降解和表面分析及其对人脐静脉内皮细胞（HUVEC）的血液相容性和细胞活力的影响。合金的静态降解速率分别为 Fe-30Mn、Fe-30Mn-1Ag 和 Fe-30Mn-3Ag 的 4.97、4.69 和 4.49mg/cm。降解后的表面分析表明，Fe-30Mn-3Ag 上形成了γ-FeOOH，而 Fe-30Mn 和 Fe-30Mn-1Ag 上则更主要是α-FeOOH。由于γ-FeOOH比α-FeOOH更易溶，因此它有助于 Fe-30Mn-3Ag 合金的进一步降解。然而，高含量的银会诱导溶血率，从而通过减少血小板黏附来抑制凝血。与 Fe-Mn 合金相比，Fe-30Mn-1Ag 和 Fe-30Mn-3Ag 合金的细胞活力得到了提高。浸泡试验后，样品的剪切屈服强度和剪切弹性模量增加，而最终剪切强度不受影响。在可接受的溶血率、低血小板黏附、可接受的细胞活力和降解后适当的机械性能的基础上，Fe-30Mn-1Ag 可以被认为是一种合适的与血液接触的可吸收铁基合金。
