Kautz Rylan, Phan Long, Arulmoli Janahan, Chatterjee Atrouli, Kerr Justin P, Naeim Mahan, Long James, Allevato Alex, Leal-Cruz Jessica E, Le LeAnn, Derakhshan Parsa, Tombola Francesco, Flanagan Lisa A, Gorodetsky Alon A
Department of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Department of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, United States.
ACS Biomater Sci Eng. 2020 Mar 9;6(3):1311-1320. doi: 10.1021/acsbiomaterials.9b00824. Epub 2020 Jan 22.
Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials. Moreover, we find that heparin selectively inhibits the adhesion of mNSPCs on reflectin, affording spatial control of cell growth and leading to a >30-fold change in cell density on patterned substrates. The described findings highlight the potential utility of reflectin as a stem cell culture material.
干细胞因其再生能力以及治疗疾病的潜力而备受关注。因此，大量的研究工作集中在开发基于蛋白质和多肽的材料作为干细胞基质和支架。在此，我们探究了头足类结构蛋白反射蛋白支持小鼠神经干/祖细胞（mNSPCs）生长的能力。我们观察到mNSPCs在反射蛋白薄膜上的黏附、生长和分化与在更成熟的基于蛋白质的材料上相当。此外，我们发现肝素选择性抑制mNSPCs在反射蛋白上的黏附，实现对细胞生长的空间控制，并导致图案化基质上的细胞密度变化超过30倍。上述发现凸显了反射蛋白作为干细胞培养材料的潜在用途。
