FialovÁ Lenka, KuchaŘ Oliver, PetrÁČkovÁ Milada, Malbohan Ivan, Zima TomÁŠ
Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.
Cent Eur J Immunol. 2020;45(2):136-143. doi: 10.5114/ceji.2020.97901. Epub 2020 Jul 27.
The heterogeneity of anti-phospholipid antibodies can be manifested not only in different antigenic specificities, but also in their avidities. The aim of the study was to investigate the relationship between anti-cardiolipin antibody (aCL) IgG avidities and levels within the range of their titres, from very low to high ones.
We analyzed 78 serum samples from 60 patients by ELISA with chaotropic agents, using urea concentration of 6 and 8 mol/l and single diluted serum samples. The changes of aCL levels and avidities were explored during a long-term follow-up in 14 patients.
The avidities of aCLs did not differ in the groups of patients classified according to aCL levels. The higher avidity antibodies predominated in our patients and the fluctuation of avidities in the longitudinal follow-up did not show significant differences. No relationship between aCL levels and their avidities was found.
aCL avidities seem to have no relationship with aCL levels and high-avidity aCLs; the potentially deleterious effects might be present also in patients with low and extremely low aCL levels. Avidity of aCLs belongs to stable characteristics with insignificant changes in time.
抗磷脂抗体的异质性不仅可体现在不同的抗原特异性上，还体现在其亲和力方面。本研究的目的是探讨抗心磷脂抗体（aCL）IgG亲和力与其滴度范围内从极低到极高水平之间的关系。
我们采用含离液剂的ELISA法，使用6mol/l和8mol/l的尿素浓度以及单稀释血清样本，分析了60例患者的78份血清样本。对14例患者进行长期随访，探讨aCL水平和亲和力的变化。
根据aCL水平分类的患者组中，aCL的亲和力没有差异。在我们的患者中，高亲和力抗体占主导，纵向随访中亲和力的波动没有显著差异。未发现aCL水平与其亲和力之间存在关联。
aCL亲和力似乎与aCL水平及高亲和力aCL无关；aCL水平低和极低的患者也可能存在潜在有害影响。aCL的亲和力属于稳定特征，随时间变化不显著。
