Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.
Thomayer University Hospital, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023 Sep;167(3):254-262. doi: 10.5507/bp.2022.003. Epub 2022 Feb 11.
Antiphosphatidylethanolamine antibodies (aPE) represent one type of antiphospholipid antibody (aPL) directed against the neutral phospholipids - phosphatidylethanolamines. The aim of this study was to evaluate levels and avidities of aPE in several groups of patients and compare them with conventional aPLs.
aPE were analysed in a cohort consisting of 68 hospitalized patients. The other cohort comprised 22 patients with immunologically-mediated diseases. The control group consisted of 20 healthy persons. ELISA methods were used for determination of aPL. Avidities of aPE were tested by modified ELISA with urea as a chaotropic agent.
aPE IgG/IgM were significantly higher in the group of patients with venous thromboembolism than those with non-thrombotic internal disorders (P=0.02 for both Ig classes). aPE IgG/IgM elevated above cut-off values were found in 10.8% of patients with venous thromboembolism and as a single aPL in 6.5%. Levels of aPE IgG higher than our limit (>6 U/mL) were detected in 29% of patients with immunologically-mediated diseases with other positive aPL. Low-, intermediate- and high-avidity aPE IgG were found in patients of both cohorts. The avidities of aPE IgG differed from those of anticardiolipin antibodies IgG. Neither aPE IgG levels nor avidity dynamics significantly changed during follow-up.
aPE may be related to venous thromboembolism and may be part of the repertoire of aPL in immunologically-mediated diseases. There are patients with thrombosis negative for conventional aPL but positive for aPE. aPE IgG may have different avidities.
抗磷脂酰乙醇胺抗体（aPE）是针对中性磷脂 - 磷脂酰乙醇胺的抗磷脂抗体（aPL）的一种类型。本研究旨在评估几种患者群体中 aPE 的水平和亲和力，并将其与常规 aPL 进行比较。
分析了由 68 名住院患者组成的队列中的 aPE。另一队列包括 22 名免疫介导性疾病患者。对照组由 20 名健康人组成。使用 ELISA 方法测定 aPL。使用尿素作为变构剂的改良 ELISA 测定 aPE 的亲和力。
静脉血栓栓塞症患者的 aPE IgG/IgM 明显高于非血栓性内部疾病患者（两种 Ig 类别的 P=0.02）。在静脉血栓栓塞症患者中，aPE IgG/IgM 升高超过临界值的比例为 10.8%，作为单一 aPL 的比例为 6.5%。在伴有其他阳性 aPL 的免疫介导性疾病患者中，aPE IgG 水平高于我们的上限（>6 U/mL）的比例为 29%。在两个队列的患者中都发现了低、中、高亲和力的 aPE IgG。aPE IgG 的亲和力与抗心磷脂抗体 IgG 不同。在随访期间，aPE IgG 水平和亲和力动力学均未发生显著变化。
aPE 可能与静脉血栓栓塞症有关，并且可能是免疫介导性疾病中 aPL 谱的一部分。有一些血栓阴性但 aPE 阳性的患者。aPE IgG 可能具有不同的亲和力。
