Exosome-based gene therapy for familial hypercholesterolemia in a mouse model.

Familial hypercholesterolemia (FH), with high LDL (low-density lipoprotein) cholesterol levels, is due to inherited mutations in genes, such as low-density lipoprotein receptor (LDLR). Development of therapeutic strategies for FH, which causes atherosclerosis and cardiovascular disease, is urgently needed. Mice with low-density lipoprotein receptor deletion ( mice) were used as an FH model. mRNA was encapsulated into exosomes by forced expression of in the donor AML12 (alpha mouse liver) cells, and the resultant exosomes were denoted as Exo. distribution of exosomes was analyzed by fluorescence labeling and imaging. The delivery efficiency of mRNA was analyzed by qPCR and Western blotting. Therapeutic effects of Exo were examined in mice by blood lipids and Oil Red O staining. The encapsulated mRNA was stable and could be translated into functional protein in the recipient cells. Following tail vein injection, exosomes were mainly delivered into the liver, producing abundant LDLR protein, resembling the endogenous expression profile in the wild-type mouse. Compared with control exosomes, Exo treatment significantly decreased lipid deposition in the liver and lowered the serum LDL-cholesterol level. Significantly, the number and size of atherosclerotic plaques and inflammation were reduced in the Exo-treated mice. We have shown that exosome-mediated mRNA delivery effectively restored receptor expression, treating the disorders in the mouse. Our study provided a new therapeutic approach for the treatment of FH patients and managing atherosclerosis.