Klein A, Lamblin G, Lhermitte M, Roussel P, Breg J, Van Halbeek H, Vliegenthart J F
Unité des Protéines, Institut National de la Santé et de la Recherche Médicale, Lille.
Eur J Biochem. 1988 Feb 1;171(3):631-42. doi: 10.1111/j.1432-1033.1988.tb13834.x.
Carbohydrate chains of respiratory-mucus glycopeptides from a patient (blood group O) suffering from bronchiectasis with a Kartagener's syndrome have been released by alkaline borohydride treatment. Application of high-performance liquid chromatography using subsequently two silica columns, one bonded with aminopropyl groups and the other with octadecyl groups, afforded 39 neutral fractions; 35 oligosaccharide-alditol structures have been characterized by employing 500-MHz 1H-NMR spectroscopy in conjunction with sugar analysis. Here, 16 oligosaccharide structures, possessing a core consisting of Gal beta(1----3)GalNAc-ol branching through a GlcNAc residue linked beta(1----6) to the GalNAc residue (core type 2 or core type 1, respectively), are described. Ten oligosaccharide-alditols with these types of cores (2, 3, 10a, 14, 7, 11a, 15a, 16a, 12 and 16c) have been identified previously in human bronchial mucins of patients suffering from cystic fibrosis [Lamblin, G., Boersma, A., Lhermitte, M., Roussel, P., Mutsaers, J.H.G.M., Van Halbeek, H. and Vliegenthart, J.F.G. (1984) Eur. J. Biochem. 143, 227-236]. Of the remaining six compounds, one is a partial structure of oligosaccharides previously described: (Formula: see text). The structures 17a and 20 contain the Y determinant, i.e., Fuc alpha(1----2)Gal beta(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----). High-resolution 1H-NMR spectroscopy is able to distinguish whether the Y determinant is beta(1----3) or beta(1----6) linked in such oligosaccharide-alditols.
通过碱性硼氢化物处理，已从一名患有支气管扩张症合并卡塔格内综合征（血型为O型）的患者的呼吸道黏液糖肽中释放出碳水化合物链。随后使用两根硅胶柱（一根键合氨丙基，另一根键合十八烷基）进行高效液相色谱分析，得到了39个中性馏分；通过结合使用500兆赫兹的1H核磁共振光谱和糖分析，已鉴定出35种低聚糖糖醇结构。在此，描述了16种低聚糖结构，其核心由通过与GalNAc残基以β(1→6)连接的GlcNAc残基分支的Galβ(1→3)GalNAc-ol组成（分别为核心类型2或核心类型1）。先前已在患有囊性纤维化的患者的人支气管黏蛋白中鉴定出十种具有这些核心类型的低聚糖糖醇（2、3、10a、14、7、11a、15a、16a、12和16c）[Lamblin, G., Boersma, A., Lhermitte, M., Roussel, P., Mutsaers, J.H.G.M., Van Halbeek, H.和Vliegenthart, J.F.G. (1984) Eur. J. Biochem. 143, 227 - 236]。在其余六种化合物中，一种是先前描述的低聚糖的部分结构：（分子式：见原文）。结构17a和20含有Y决定簇，即Fucα(1→2)Galβ(1→4)[Fucα(1→3)]GlcNAcβ(1→)。高分辨率1H核磁共振光谱能够区分在这种低聚糖糖醇中Y决定簇是β(1→3)还是β(1→6)连接。
