基于单分子实时测序技术的食用菌线粒体基因组
SMRT-based mitochondrial genome of the edible mushroom .
作者信息
Li Wei, Zhang Fen, Gao Li-Zhi
机构信息
Institution of Genomics and Bioinformatics, South China Agricultural University, Guangzhou, China.
Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwestern China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
出版信息
Mitochondrial DNA B Resour. 2020 Aug 25;5(3):3201-3202. doi: 10.1080/23802359.2020.1810160.
Pers. is a highly-prized mushroom for its edible and medical values. In this study, we determined the complete mitochondrial genome of combining both PacBio and Illumina sequencing technologies. The complete mitochondrial genome is 280,763 bp in length with a GC content of 39.88%. We identified a total of 14 core conserved protein-coding genes, 127 non-conserved open reading frames (ncORFs) and 30 tRNA genes in the mitogenome. However, no large or small rRNA subunits (rnl or rns) were identified in this mitogenome. In addition, we detected two mitochondrial RNase P (rnpB) genes and one ribosomal protein genes (rps3). Phylogenetic analysis was performed among and 18 other representative fungi from Ascomycota, Basidiomycota and Mucoromycota. Our results showed that was most closely related to . The availability of the mitochondrial genome will form the basis of genetic breeding program and enhance our understanding of the evolution of this species.
Pers.是一种因其食用和药用价值而备受珍视的蘑菇。在本研究中，我们结合PacBio和Illumina测序技术确定了Pers.的完整线粒体基因组。完整的线粒体基因组长度为280,763 bp，GC含量为39.88%。我们在该线粒体基因组中总共鉴定出14个核心保守蛋白编码基因、127个非保守开放阅读框（ncORFs）和30个tRNA基因。然而，在该线粒体基因组中未鉴定出大或小核糖体RNA亚基（rnl或rns）。此外，我们检测到两个线粒体核糖核酸酶P（rnpB）基因和一个核糖体蛋白基因（rps3）。在Pers.与来自子囊菌门、担子菌门和毛霉门的其他18种代表性真菌之间进行了系统发育分析。我们的结果表明Pers.与[具体物种名称]关系最为密切。Pers.线粒体基因组的可用性将为遗传育种计划奠定基础，并增进我们对该物种进化的理解。
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