Hussain Saddam, Bukhari Syed Mohsin, Javid Arshad, Hussain Ali, Rashid Muhammad, Ali Waqas
Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Mitochondrial DNA B Resour. 2020 Aug 26;5(3):3218-3220. doi: 10.1080/23802359.2020.1810143.
The Indus valley toad and common Asian toad are widely distributed toads in Pakistan. There is doubt in the taxonomic position of species within the genus in Pakistan as most of the species identified on morphology. Previously, identified on morphology but during the present study, it is confirmed as based COI sequences (MK941836). The interspecific divergence between and was 16%. The intraspecific divergence of (MK947909.1) was ranging from 0% to 1% while the intraspecific divergence of (MK941836) was high ranging from 10% to 11%. Overall, genetic variation between the species of genus based on p-distance was 14%. In our recommendation, a large-scale molecular identification of amphibians should take into consideration for exact species identification to report any new species from Pakistan.
印度河蟾蜍和亚洲普通蟾蜍是巴基斯坦分布广泛的蟾蜍。巴基斯坦该属物种的分类地位存在疑问，因为大多数物种是基于形态学鉴定的。此前是基于形态学鉴定的，但在本研究中，根据细胞色素氧化酶亚基I（COI）序列（MK941836）得到了确认。[此处原文未明确提及的两个物种名称]之间的种间差异为16%。[此处原文未明确提及的一个物种名称，其COI序列为MK947909.1]的种内差异为0%至1%，而[此处原文未明确提及的另一个物种名称，其COI序列为MK941836]的种内差异较高，为10%至11%。总体而言，基于p距离，该属物种之间的遗传变异为14%。我们建议，为了准确鉴定物种以报告来自巴基斯坦的任何新物种，应考虑对两栖动物进行大规模分子鉴定。
