Molecular identification of genus from Punjab, Pakistan.

The Indus valley toad and common Asian toad are widely distributed toads in Pakistan. There is doubt in the taxonomic position of species within the genus in Pakistan as most of the species identified on morphology. Previously, identified on morphology but during the present study, it is confirmed as based COI sequences (MK941836). The interspecific divergence between and was 16%. The intraspecific divergence of (MK947909.1) was ranging from 0% to 1% while the intraspecific divergence of (MK941836) was high ranging from 10% to 11%. Overall, genetic variation between the species of genus based on p-distance was 14%. In our recommendation, a large-scale molecular identification of amphibians should take into consideration for exact species identification to report any new species from Pakistan.