Ali Waqas, Javid Arshad, Hussain Ali, Hafeez-Ur-Rehman Muhammad, Chabber Anne-Lise, Hemmatzadeh Farid
Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Mitochondrial DNA B Resour. 2020 Feb 28;5(2):1227-1231. doi: 10.1080/23802359.2020.1731337. eCollection 2020.
The present study documented the first record of from Punjab, Pakistan. The specimens were collected during field visits from June through August 2018. Various morphometric measurements of were taken and compared with Snout-vent length (SVL) was 38.11 ± 0.87 mm ( = 5), snout length was 3% of SVL, foot length was 55% of SVL, head length was 32% of SVL and weight was 8.01 ± 0.12 g ( = 5). A few specimens ( = 2) were euthanized and preserved for molecular analysis through mitochondrial 16S rRNA gene sequences. The newly obtained DNA sequences of were submitted to GenBank and accession numbers were obtained (MK881165.1 and MK920114.1). The Maximum likelihood and Neighbor-joining trees based on Kimura 2-parameter distance resulted in similar phylogenetic trees. was out group in both phylogenetic trees. The interspecific divergence of and was high ranging from 4% to 6% as compared to low intraspecific divergence 0% and 1%. The diversity and distribution ranges of many amphibians species are not well known in Pakistan due to lack of taxonomic information. In our recommendation, a large scale DNA barcoding is required to report more cryptic or new species from Pakistan.
本研究记录了来自巴基斯坦旁遮普邦的首例[物种名称]记录。标本于2018年6月至8月的实地考察期间采集。对[物种名称]进行了各种形态测量，并与[对比物种名称]进行了比较。吻肛长度（SVL）为38.11±0.87毫米（n = 5），吻长为SVL的3%，足长为SVL的55%，头长为SVL的32%，体重为8.01±0.12克（n = 5）。几只标本（n = 2）被安乐死并保存下来，用于通过线粒体16S rRNA基因序列进行分子分析。新获得的[物种名称]DNA序列已提交至GenBank并获得了登录号（MK881165.1和MK920114.1）。基于Kimura双参数距离的最大似然树和邻接树产生了相似的系统发育树。[对比物种名称]在两个系统发育树中均为外类群。[物种名称]与[对比物种名称]的种间差异较高，范围为4%至6%，而种内差异较低，为0%和1%。由于缺乏分类学信息，巴基斯坦许多两栖动物物种的多样性和分布范围尚不清楚。我们建议，需要进行大规模的DNA条形码分析，以报告来自巴基斯坦的更多隐秘或新物种。
