Shrestha Sandeep, Bashyal Ashish, Dhakal Ashna, McGreevy Thomas J, Buffum Bill, Joshi Jyoti, Chaudhary Hemanta Kumari, Khanal Sanjay Nath
Department of Environmental Science and Engineering, Kathmandu University, Kavrepalanchok, Nepal.
Biodiversity Conservancy Nepal, Butwal, Nepal.
Mitochondrial DNA B Resour. 2020 Aug 31;5(3):3257-3261. doi: 10.1080/23802359.2020.1811174.
Chinese Pangolins () are Critically Endangered and one of the most illegally traded mammals globally. We generated first COI sequences from five individuals of this species from Nepal. BLASTn search of our 600 bp sequences at GenBank showed pair-wise identity between 99.17% and 100% to . There were three haplotypes and a total of five variable sites among five sequences. Neighbor-joining tree revealed that all . from Nepal clustered into same group further splitting into two sub-groups albeit with low bootstrap value, suggesting potential multiple geographic origins. The K2P distance was 0.3% within group and 0.7% between four sequences from Bhaktapur and Kavrepalanchok districts (Mape2, Mape3, Mape5 and Mape6) and museum sample (Mape10). This study has generated reference samples for from Nepal and will be helpful in understanding dynamics of illegal trade of this species and in successful identification of from Nepal even in the absence of intact specimens.
中华穿山甲极度濒危，是全球非法交易最为频繁的哺乳动物之一。我们从尼泊尔的5只中华穿山甲个体中首次获得了细胞色素氧化酶亚基I（COI）序列。在GenBank数据库中对我们600bp的序列进行BLASTn搜索发现，其与[具体物种]的序列两两之间的一致性在99.17%至100%之间。在5条序列中有3种单倍型和总共5个可变位点。邻接树显示，来自尼泊尔的所有中华穿山甲都聚集在同一组中，尽管自展值较低，但进一步分为两个亚组，这表明可能存在多个地理起源。组内的K2P距离为0.3%，来自巴克塔普尔和卡弗勒帕兰乔克地区的4条序列（Mape2、Mape3、Mape5和Mape6）与博物馆样本（Mape10）之间的K2P距离为0.7%。本研究为尼泊尔的中华穿山甲生成了参考样本，将有助于了解该物种非法交易的动态情况，即使在没有完整标本的情况下，也有助于成功鉴定尼泊尔的中华穿山甲。
