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利用DNA条形码技术从没收的穿山甲残骸中确定物种身份。

Determining species identity from confiscated pangolin remains using DNA barcoding.

作者信息

Luczon Adrian U, Ong Perry S, Quilang Jonas P, Fontanilla Ian Kendrich C

机构信息

Institute of Biology, University of the Philippines, Diliman, Quezon City, Philippines.

出版信息

Mitochondrial DNA B Resour. 2016 Oct 18;1(1):763-766. doi: 10.1080/23802359.2016.1238752.

Abstract

Illegal wildlife trade is one of the key threats to biodiversity. A requisite in combating illegal wildlife trade is through effective and efficient identification of confiscated wildlife or wildlife remains. This can be done through DNA barcoding. In this study, DNA barcoding was employed on several cases of poaching in the Philippines involving 85 unidentified pangolin remains. Of these, 73 specimens confiscated from Palawan were identified as the Palawan endemic , but no deep divergences were observed, suggesting that the samples originated from a single locality. The other 12 individuals, which were part of a large haul of pangolin carcasses recovered from a foreign fishing vessel that ran aground in Tubattaha Reefs, Philippines, were identified as the Malayan Pangolin, . They split into two groups with 3.3% mean genetic distance, suggesting at least two geographic origins.

摘要

非法野生动物贸易是生物多样性面临的主要威胁之一。打击非法野生动物贸易的一个必要条件是有效且高效地识别被没收的野生动物或野生动物残骸。这可以通过DNA条形码技术来实现。在本研究中,DNA条形码技术被应用于菲律宾几起偷猎案件,涉及85具身份不明的穿山甲残骸。其中,从巴拉望岛没收的73个标本被鉴定为巴拉望岛特有种,但未观察到明显的分化,这表明样本来自单一地点。另外12只穿山甲是从一艘在菲律宾图巴塔哈礁搁浅的外国渔船上查获的大量穿山甲尸体的一部分,被鉴定为马来穿山甲。它们分成两组,平均遗传距离为3.3%,这表明至少有两个地理起源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b3/7800584/ce686dc1b2a3/TMDN_A_1238752_F0001_B.jpg

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