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Psoralen photofootprinting of protein-binding sites on DNA.

作者信息

Zhen W P, Jeppesen C, Nielsen P E

机构信息

Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.

出版信息

FEBS Lett. 1988 Feb 29;229(1):73-6. doi: 10.1016/0014-5793(88)80800-7.

DOI:10.1016/0014-5793(88)80800-7
PMID:3345840
Abstract

Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

摘要

相似文献

1
Psoralen photofootprinting of protein-binding sites on DNA.
FEBS Lett. 1988 Feb 29;229(1):73-6. doi: 10.1016/0014-5793(88)80800-7.
2
Site specificity of psoralen-DNA interstrand cross-linking determined by nuclease Bal31 digestion.
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3
Hyperreactivity of B-Z junctions to 4,5',8-trimethylpsoralen photobinding assayed by an exonuclease III/photoreversal mapping procedure.通过核酸外切酶III/光逆转映射程序测定B-Z连接对4,5',8-三甲基补骨脂素光结合的高反应性。
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Photoaddition of trimethylpsoralen as a probe for the intracellular organization of Escherchia coli DNA.作为研究大肠杆菌DNA细胞内组织的探针，三甲基补骨脂素的光加成反应
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Uranyl photofootprinting.铀酰光足迹法
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The effects of covalent additions of a psoralen on transcription by E. coli RNA polymerase.补骨脂素的共价加成对大肠杆菌RNA聚合酶转录的影响。
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8
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DNA recognition by the helix-turn-helix motif: investigation by laser Raman spectroscopy of the phage lambda repressor and its interaction with operator sites OL1 and OR3.通过螺旋-转角-螺旋基序进行的DNA识别：利用激光拉曼光谱对噬菌体λ阻遏物及其与操纵子位点OL1和OR3的相互作用进行研究。
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10
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Nucleic Acids Res. 1989 May 25;17(10):3747-55. doi: 10.1093/nar/17.10.3747.

引用本文的文献

1
High resolution footprinting of EcoRI and distamycin with Rh(phi)2(bpy)3+, a new photofootprinting reagent.用新型光足迹试剂Rh(phi)2(bpy)3+对EcoRI和Distamycin进行高分辨率足迹分析。
Nucleic Acids Res. 1989 Dec 25;17(24):10259-79. doi: 10.1093/nar/17.24.10259.
2
Uranyl mediated photofootprinting reveals strong E. coli RNA polymerase--DNA backbone contacts in the +10 region of the DeoP1 promoter open complex.铀酰介导的光足迹法揭示了DeoP1启动子开放复合物+10区域中大肠杆菌RNA聚合酶与DNA主链之间的紧密接触。
Nucleic Acids Res. 1989 Jul 11;17(13):4947-56. doi: 10.1093/nar/17.13.4947.
3
Light affects the structure of Chlamydomonas chloroplast chromosomes.
光会影响衣藻叶绿体染色体的结构。
Nucleic Acids Res. 1990 May 11;18(9):2625-31. doi: 10.1093/nar/18.9.2625.
4
Intercalation of psoralen into DNA of plastid chromosomes decreases late during barley chloroplast development.补骨脂素嵌入质体染色体的DNA在大麦叶绿体发育后期减少。
Nucleic Acids Res. 1991 Oct 11;19(19):5219-25. doi: 10.1093/nar/19.19.5219.