Hallick L M, Hanson C V, Cacciapuoti J O, Hearst J E
Nucleic Acids Res. 1980 Feb 11;8(3):611-22. doi: 10.1093/nar/8.3.611.
It has been previously demonstrated that photoreaction with 4,5',8-tri-methylpsoralen (trioxsalen) can be used as a probe for the in vivo structure of eucaryotic chromatin. We have used this probe to analyze the organization of intracellular Escherichia coli DNA. In contrast to eucaryotic DNA, bacterial DNA within the intact cell is not protected from saturating doses of trioxsalen photoaddition. The final level of covalently bound trioxsalen upon saturation is identical to that found with purified DNA. In addition, the distribution of interstrand DNA cross-links formed by low doses of trioxsalen photoadducts does not exhibit the repeating pattern that has been observed with eucaryotic nucleosomes.
先前已经证明，4,5',8-三甲基补骨脂素（三甲沙林）的光反应可作为真核染色质体内结构的探针。我们已使用该探针分析细胞内大肠杆菌DNA的组织情况。与真核DNA不同，完整细胞内的细菌DNA无法免受饱和剂量三甲沙林光加成的影响。饱和时共价结合的三甲沙林的最终水平与纯化DNA的情况相同。此外，低剂量三甲沙林光加合物形成的链间DNA交联分布未呈现出真核核小体所观察到的重复模式。
