Asif Muhammad Zaka, Van der Gaag Victoria L, Guo Jane, Nocilla Kelsey A, Muzio Cole J, Edison Arthur S
Department of Biochemistry & Molecular Biology and Complex Carbohydrate Research Center, University of Georgia.
Department of Genetics.
MicroPubl Biol. 2021 Jan 13;2021. doi: 10.17912/micropub.biology.000352.
is an ideal model organism for studying the xenobiotic detoxification pathways of various natural and synthetic toxins. We developed a new workflow to study the effects of 1-hydroxyphenazine (1-HP), a toxin produced by the bacterium , on the survival of . . Prior research has demonstrated that can detoxify 1-HP through the general mechanism of O-glycosylation. As part of the Vertically Integrated Projects (VIP) undergraduate research team, we have developed a workflow for a plate-based toxicity assay to determine the median lethal dose (LD50) of 1-HP. This was achieved through a toxin exposure method in which the worms were exposed to varying concentrations of 1-HP. The death rates were measured using a fluorescent bead assay. This workflow can be used to test . responses to different toxins and also the response of different mutant strains to a toxin of interest.
是研究各种天然和合成毒素的异生物质解毒途径的理想模式生物。我们开发了一种新的工作流程，以研究由细菌产生的毒素1-羟基吩嗪（1-HP）对……生存的影响。先前的研究表明，……可以通过O-糖基化的一般机制对1-HP进行解毒。作为垂直整合项目（VIP）本科研究团队的一部分，我们开发了一种基于平板的毒性测定工作流程，以确定1-HP的半数致死剂量（LD50）。这是通过一种毒素暴露方法实现的，其中将蠕虫暴露于不同浓度的1-HP。使用荧光珠测定法测量死亡率。该工作流程可用于测试……对不同毒素的反应，以及不同突变菌株对感兴趣毒素的反应。
