• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

UDP-糖基转移酶（）基因在[具体物种]中对1-羟基吩嗪（1-HP）的解毒和糖基化作用

Role of UDP-Glycosyltransferase () Genes in Detoxification and Glycosylation of 1-Hydroxyphenazine (1-HP) in .

作者信息

Asif Muhammad Zaka, Nocilla Kelsey A, Ngo Li, Shah Man, Smadi Yosef, Hafeez Zaki, Parnes Michael, Manson Allie, Glushka John N, Leach Franklin E, Edison Arthur S

机构信息

Department of Biochemistry & Molecular Biology, University of Georgia, Athens, Georgia 30602, United States.

Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, United States.

出版信息

Chem Res Toxicol. 2024 Apr 15;37(4):590-599. doi: 10.1021/acs.chemrestox.3c00410. Epub 2024 Mar 15.

DOI:10.1021/acs.chemrestox.3c00410
PMID:38488606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11022241/
Abstract

is a useful model organism to study the xenobiotic detoxification pathways of various natural and synthetic toxins, but the mechanisms of phase II detoxification are understudied. 1-Hydroxyphenazine (1-HP), a toxin produced by the bacterium , kills . We previously showed that detoxifies 1-HP by adding one, two, or three glucose molecules in N2 worms. Our current study evaluates the roles that some UDP-glycosyltransferase () genes play in 1-HP detoxification. We show that and knockout mutants are more sensitive to 1-HP than reference strains N2 or PD1074. Our data also show that knockout mutants produce reduced amounts of the trisaccharide sugars, while the knockout mutants produce reduced amounts of all 1-HP derivatives except for the glucopyranosyl product compared to the reference strains. We characterized the structure of the trisaccharide sugar phenazines made by and showed that one of the sugar modifications contains an -acetylglucosamine (GlcNAc) in place of glucose. This implies broad specificity regarding UGT function and the role of genes other than in adding GlcNAc, at least in small-molecule detoxification.

摘要

是研究各种天然和合成毒素的异生物质解毒途径的有用模式生物，但II期解毒机制的研究较少。1-羟基吩嗪（1-HP）是由细菌产生的一种毒素，可杀死。我们之前表明，在N2蠕虫中通过添加一个、两个或三个葡萄糖分子来解毒1-HP。我们目前的研究评估了一些尿苷二磷酸糖基转移酶（）基因在1-HP解毒中的作用。我们表明，与参考菌株N2或PD1074相比，和敲除突变体对1-HP更敏感。我们的数据还表明，与参考菌株相比，敲除突变体产生的三糖量减少，而敲除突变体除了吡喃葡萄糖基产物外，所有1-HP衍生物的产量都减少。我们对和产生的三糖吩嗪的结构进行了表征，并表明其中一种糖修饰含有N-乙酰葡糖胺（GlcNAc）代替葡萄糖。这意味着UGT功能具有广泛的特异性，并且除了在添加GlcNAc方面的作用外，至少在小分子解毒中，其他基因也发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/ce75a8c4b34f/tx3c00410_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/44cea9545ad7/tx3c00410_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/62a635fabd26/tx3c00410_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/77064ac0f87a/tx3c00410_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/33ad10cb4775/tx3c00410_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/37c87472e450/tx3c00410_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/ce75a8c4b34f/tx3c00410_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/44cea9545ad7/tx3c00410_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/62a635fabd26/tx3c00410_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/77064ac0f87a/tx3c00410_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/33ad10cb4775/tx3c00410_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/37c87472e450/tx3c00410_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83d0/11022241/ce75a8c4b34f/tx3c00410_0006.jpg

相似文献

1
Role of UDP-Glycosyltransferase () Genes in Detoxification and Glycosylation of 1-Hydroxyphenazine (1-HP) in .UDP-糖基转移酶（）基因在[具体物种]中对1-羟基吩嗪（1-HP）的解毒和糖基化作用
Chem Res Toxicol. 2024 Apr 15;37(4):590-599. doi: 10.1021/acs.chemrestox.3c00410. Epub 2024 Mar 15.
2
Multiple UDP glycosyltransferases modulate benzimidazole drug sensitivity in the nematode Caenorhabditis elegans in an additive manner.多种 UDP 糖基转移酶以累加的方式调节线虫秀丽隐杆线虫对苯并咪唑类药物的敏感性。
Int J Parasitol. 2024 Sep;54(11):535-549. doi: 10.1016/j.ijpara.2024.05.003. Epub 2024 May 26.
3
UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences.旋毛虫属 UDP-糖基转移酶家族：系统发育分析、组成型表达、性别差异和耐药相关差异。
Int J Parasitol Drugs Drug Resist. 2018 Dec;8(3):420-429. doi: 10.1016/j.ijpddr.2018.09.005. Epub 2018 Sep 26.
4
A Plate Based Assay for Determination of the Median Lethal Dose of 1-Hydroxyphenazine in .一种基于平板的测定1-羟基吩嗪在……中半数致死剂量的试验方法 。 （你提供的原文中“in”后面内容不完整）
MicroPubl Biol. 2021 Jan 13;2021. doi: 10.17912/micropub.biology.000352.
5
Glucose or Altered Ceramide Biosynthesis Mediate Oxygen Deprivation Sensitivity Through Novel Pathways Revealed by Transcriptome Analysis in Caenorhabditis elegans.葡萄糖或改变的神经酰胺生物合成通过秀丽隐杆线虫转录组分析揭示的新途径介导缺氧敏感性。
G3 (Bethesda). 2016 Oct 13;6(10):3149-3160. doi: 10.1534/g3.116.031583.
6
Identification and tissue expression profiling of candidate UDP-glycosyltransferase genes expressed in Holotrichia parallela motschulsky antennae.暗黑鳃金龟触角中表达的候选尿苷二磷酸糖基转移酶基因的鉴定及组织表达谱分析
Bull Entomol Res. 2018 Dec;108(6):807-816. doi: 10.1017/S0007485318000068. Epub 2018 Feb 5.
7
Bacterial origin of a diverse family of UDP-glycosyltransferase genes in the Tetranychus urticae genome.二斑叶螨基因组中一个多样的UDP-糖基转移酶基因家族的细菌起源
Insect Biochem Mol Biol. 2014 Jul;50:43-57. doi: 10.1016/j.ibmb.2014.04.003. Epub 2014 Apr 13.
8
Structural dissection of unnatural ginsenoside-biosynthetic UDP-glycosyltransferase Bs-YjiC from Bacillus subtilis for substrate promiscuity.来自枯草芽孢杆菌的非天然人参皂苷生物合成UDP-糖基转移酶Bs-YjiC的结构剖析以研究底物选择性。
Biochem Biophys Res Commun. 2021 Jan 1;534:73-78. doi: 10.1016/j.bbrc.2020.11.104. Epub 2020 Dec 10.
9
Identification of residues that confer sugar selectivity to UDP-glycosyltransferase 3A (UGT3A) enzymes.鉴定赋予 UDP-糖基转移酶 3A（UGT3A）酶糖选择性的残基。
J Biol Chem. 2012 Jul 13;287(29):24122-30. doi: 10.1074/jbc.M112.343608. Epub 2012 May 23.
10
Genome Wide Analysis of Family-1 UDP Glycosyltransferases in Specifies Abiotic Stress Responsive Glycosylation Mechanisms.全家族 1 UDP 糖基转移酶的基因组分析揭示了非生物胁迫响应性糖基化机制。
Genes (Basel). 2022 Sep 13;13(9):1640. doi: 10.3390/genes13091640.

引用本文的文献

1
Transcriptomic analysis reveals molecular mechanism by which Chinese olive fruit prolongs lifespan of Caenorhabditis elegans.转录组学分析揭示了油橄榄果实延长秀丽隐杆线虫寿命的分子机制。
NPJ Sci Food. 2025 May 30;9(1):90. doi: 10.1038/s41538-025-00456-1.
2
Glycosylation in T2 high and Th17 Asthma: A Narrative Review.T2高型和Th17型哮喘中的糖基化：一篇叙述性综述
J Asthma Allergy. 2025 Apr 12;18:545-558. doi: 10.2147/JAA.S509940. eCollection 2025.
3
Molecular mechanism of culinary herb Artemisia argyi in promoting lifespan and stress tolerance.

本文引用的文献

1
Computational analysis of variation in .……变异的计算分析。 （你提供的原文不完整，这里是补充完整后的大致意思，具体翻译需根据完整原文准确进行。）
MicroPubl Biol. 2023 Aug 7;2023. doi: 10.17912/micropub.biology.000819. eCollection 2023.
2
The Glycosyltransferase Pathway: An Integrated Analysis of the Cell Metabolome.糖基转移酶途径：细胞代谢组的综合分析
Metabolites. 2022 Oct 21;12(10):1006. doi: 10.3390/metabo12101006.
3
Regulation of human UDP-glycosyltransferase () genes by miRNAs.miRNAs 对人类 UDP-糖基转移酶 () 基因的调控。
烹饪香草艾叶促进寿命和应激耐受性的分子机制。
NPJ Sci Food. 2024 Dec 24;8(1):111. doi: 10.1038/s41538-024-00358-8.
4
Recent Advances in Phenazine Natural Products: Chemical Structures and Biological Activities.嗪类天然产物的最新进展：化学结构和生物活性。
Molecules. 2024 Oct 9;29(19):4771. doi: 10.3390/molecules29194771.
5
Computational analysis of variation in .……变异的计算分析。 （你提供的原文不完整，这里是补充完整后的大致意思，具体翻译需根据完整原文准确进行。）
MicroPubl Biol. 2023 Aug 7;2023. doi: 10.17912/micropub.biology.000819. eCollection 2023.
Drug Metab Rev. 2022 May;54(2):120-140. doi: 10.1080/03602532.2022.2048846. Epub 2022 Mar 11.
4
Complete H and C NMR chemical shift assignments of mono-to tetrasaccharides as basis for NMR chemical shift predictions of oligo- and polysaccharides using the computer program CASPER.完成单糖至四糖的 1H 和 13C NMR 化学位移赋值，为使用计算机程序 CASPER 预测寡糖和多糖的 NMR 化学位移提供基础。
Carbohydr Res. 2022 Mar;513:108528. doi: 10.1016/j.carres.2022.108528. Epub 2022 Feb 25.
5
Biosynthesis and metabolic engineering of 1-hydroxyphenazine in Pseudomonas chlororaphis H18.在假单胞菌 H18 中 1-羟基吩嗪的生物合成和代谢工程。
Microb Cell Fact. 2021 Dec 30;20(1):235. doi: 10.1186/s12934-021-01731-y.
6
Long-Term Metabolomics Reference Material.长期代谢组学参考物质。
Anal Chem. 2021 Jul 6;93(26):9193-9199. doi: 10.1021/acs.analchem.1c01294. Epub 2021 Jun 22.
7
Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.大规模混合阶段秀丽隐杆线虫群体的培养和分析。
J Vis Exp. 2021 May 5(171). doi: 10.3791/61453.
8
Xenobiotic metabolism and transport in .外源物质代谢与转运。
J Toxicol Environ Health B Crit Rev. 2021 Feb 17;24(2):51-94. doi: 10.1080/10937404.2021.1884921. Epub 2021 Feb 22.
9
A Plate Based Assay for Determination of the Median Lethal Dose of 1-Hydroxyphenazine in .一种基于平板的测定1-羟基吩嗪在……中半数致死剂量的试验方法 。 （你提供的原文中“in”后面内容不完整）
MicroPubl Biol. 2021 Jan 13;2021. doi: 10.17912/micropub.biology.000352.
10
Similarities and differences in the biotransformation and transcriptomic responses of Caenorhabditis elegans and Haemonchus contortus to five different benzimidazole drugs.秀丽隐杆线虫和捻转血矛线虫对五种不同苯并咪唑类药物的生物转化和转录组反应的异同。
Int J Parasitol Drugs Drug Resist. 2019 Dec;11:13-29. doi: 10.1016/j.ijpddr.2019.09.001. Epub 2019 Sep 9.