Camargo Sergio, Leshkowitz Dena, Dassa Bareket, Mariscal Vicente, Flores Enrique, Stavans Joel, Arbel-Goren Rinat
Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel.
Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 76100, Israel.
iScience. 2021 Jan 5;24(1):101977. doi: 10.1016/j.isci.2020.101977. eCollection 2021 Jan 22.
Cell-cell communication is an essential attribute of multicellular organisms. The effects of perturbed communication were studied in septal protein mutants of the heterocyst-forming filamentous cyanobacterium sp. PCC 7120 model organism. Strains bearing and deletions showed differences in growth, pigment absorption spectra, and spatial patterns of expression of the gene encoding a heterocyst differentiation master regulator. Global changes in gene expression resulting from deletion of those genes were mapped by RNA sequencing analysis of wild-type and mutant strains, both under nitrogen-replete and nitrogen-poor conditions. The effects of and deletions were non-additive, and perturbed cell-cell communication led to significant changes in global gene expression. Most significant effects, related to carbon metabolism, included increased expression of genes encoding carbon uptake systems and components of the photosynthetic apparatus, as well as decreased expression of genes encoding cell wall components related to heterocyst differentiation and to polysaccharide export.
细胞间通讯是多细胞生物的一个基本属性。在形成异形胞的丝状蓝细菌模式生物集胞藻PCC 7120的隔膜蛋白突变体中研究了通讯紊乱的影响。携带缺失和缺失的菌株在生长、色素吸收光谱以及编码异形胞分化主调节因子的基因的空间表达模式上存在差异。通过对野生型和突变体菌株在富氮和贫氮条件下的RNA测序分析，绘制了因这些基因缺失而导致的基因表达全局变化。缺失和缺失的影响是非累加性的，细胞间通讯紊乱导致全局基因表达发生显著变化。与碳代谢相关的最显著影响包括编码碳摄取系统和光合装置组件的基因表达增加，以及编码与异形胞分化和多糖输出相关的细胞壁组件的基因表达减少。
