通过磷酸化 MLKL 免疫组织化学标记检测细胞坏死性凋亡。
Detection of necroptosis by phospho-MLKL immunohistochemical labeling.
机构信息
State Key Laboratory of Cellular Stress Biology, Xiang'an Hospital of Xiamen University, Cancer Research Center of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian 361102, China.
Research Unit of Cellular Stress of CAMS, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.
出版信息
STAR Protoc. 2020 Dec 30;2(1):100251. doi: 10.1016/j.xpro.2020.100251. eCollection 2021 Mar 19.
TNF-induced necroptosis is involved in many physiological and pathological processes. Phospho-MLKL is a hallmark of necroptosis. Cecum is a sensitive organ with extensive necroptosis responses to TNF . Here, taking advantage of commercially available mouse TNF and easily accessible reagents and materials, we systematically provide a detailed and highly versatile protocol of detecting necroptosis signaling in mouse cecum by immunohistochemical labeling, which can also be used in other tissues or antibodies in immunohistochemical staining. For complete details on the use and execution of this protocol, please refer to Yang et al. (2020) and Chen et al. (2015).
TNF 诱导的细胞坏死性凋亡参与许多生理和病理过程。磷酸化-MLKL 是细胞坏死性凋亡的一个标志。盲肠是一个敏感的器官，对 TNF 有广泛的坏死性凋亡反应。在这里，我们利用商业上可获得的小鼠 TNF 以及易于获得的试剂和材料，通过免疫组织化学标记系统地提供了一种检测小鼠盲肠中坏死性凋亡信号的详细且高度通用的方案，该方案也可用于其他组织或免疫组织化学染色中的抗体。有关该方案使用和执行的完整详细信息，请参见 Yang 等人（2020 年）和 Chen 等人（2015 年）。
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