Center for Clinical Trial and Research Support, Fujita Health University Research Promotion and Support Headquarters, Toyoake, Aichi, 470-1192, Japan.
Graduate School of Medicine, Fujita Health University, Toyoake, Aichi, 470-1192, Japan.
Med Mol Morphol. 2021 Sep;54(3):216-226. doi: 10.1007/s00795-021-00280-y. Epub 2021 Jan 17.
The incidence rate of post-cataract surgery posterior capsule opacification (PCO) and lens turbidity is about 20% in 5 years. Soemmering's ring, which is a type of PCO also called a regenerated lens with similar tissue structure to that of a human lens, is an important proxy for elucidating the mechanism of lens regeneration and maintenance of transparency. The authors created new human immortalized crystalline lens epithelial cells (iHLEC-NY1s) with excellent differentiation potential, and as a result of culturing the cells by static and rotation-floating methods, succeeded in producing a three-dimensional cell structure model (3D-iHLEC-NY1s) which is similar to Soemmering's ring in tissue structure and expression characteristics of αA-crystalline, βB2-crystalline, vimentin proteins. 3D-iHLEC-NY1s is expected to be a proxy in vitro experimental model of Soemmering's ring to enable evaluation of drug effects on suppression of cell aggregate formation and transparency. By further improving the culture conditions, we aim to control the cell sequence and elucidate the mechanism underlying the maintenance of lens transparency.
白内障手术后后囊混浊（PCO）和晶状体混浊的发生率约为 5 年内的 20%。Soemmering 环是 PCO 的一种类型，也称为具有与人晶状体相似组织结构的再生晶状体，是阐明晶状体再生和维持透明度机制的重要替代物。作者创建了具有出色分化潜力的新型人永生化晶状体上皮细胞（iHLEC-NY1s），通过静态和旋转浮法培养细胞，成功地产生了类似于 Soemmering 环的三维细胞结构模型（3D-iHLEC-NY1s）在组织结构和αA-晶状体、βB2-晶状体、波形蛋白蛋白的表达特征方面。3D-iHLEC-NY1s 有望成为 Soemmering 环的体外实验模型，以评估药物抑制细胞聚集形成和透明度的效果。通过进一步改善培养条件，我们旨在控制细胞序列并阐明维持晶状体透明度的机制。
