爱尔兰家猫中猫白血病病毒和猫免疫缺陷病毒的流行情况。
Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland.
机构信息
1Department of Pathology, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary.
2Centre for Bioinformatics, University of Veterinary Medicine, Budapest, Hungary.
出版信息
Acta Vet Hung. 2021 Jan 16;68(4):413-420. doi: 10.1556/004.2020.00056.
Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting felid species worldwide. A study was performed over a period of 5 months in Ireland with the aim to get an updated and more realistic prevalence of these retroviruses. A total of 183 EDTA-anticoagulated whole-blood samples were collected from cats distributed between 10 clinics. The samples were tested using both point-of-care enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Basic clinical data and vaccination history were also recorded for the sampled cats. The results of ELISA tests showed a prevalence of 10.4 and 3.3% for FIV and FeLV, respectively, and an apparent prevalence of 9.3% for FIV and 11.6% for FeLV with PCR. Phylogenetic analysis of the partial polymerase (pol) gene sequences obtained from 8 FIV-positive strains showed that all but one of the Irish strains belonged to FIV subtype A, and one to subtype B. The overall mean genetic similarity between the analysed strains was 91.15%.
猫免疫缺陷病毒（FIV）和猫白血病病毒（FeLV）是影响全球猫科动物的逆转录病毒。在爱尔兰进行了为期 5 个月的研究，旨在获得这些逆转录病毒更更新和更现实的流行率。共从 10 家诊所分布的猫中采集了 183 份 EDTA 抗凝全血样本。使用即时检测酶联免疫吸附试验（ELISA）和聚合酶链反应（PCR）对样本进行了检测。还记录了采样猫的基本临床数据和疫苗接种史。ELISA 检测结果显示，FIV 和 FeLV 的流行率分别为 10.4%和 3.3%，PCR 检测的 FIV 和 FeLV 的表观流行率分别为 9.3%和 11.6%。从 8 株 FIV 阳性株获得的部分聚合酶（pol）基因序列的系统发育分析表明，除一株外，所有爱尔兰株均属于 FIV 亚型 A，一株属于亚型 B。分析株之间的总体平均遗传相似度为 91.15%。
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