Szilasi Anna, Dénes Lilla, Krikó Eszter, Heenemann Kristin, Ertl Reinhard, Mándoki Míra, Vahlenkamp Thomas W, Balka Gyula
Department of Pathology, University of Veterinary Medicine, Budapest, Pest, Hungary.
Centre for Bioinformatics, University of Veterinary Medicine, Budapest, Pest, Hungary.
JFMS Open Rep. 2019 Dec 10;5(2):2055116919892094. doi: 10.1177/2055116919892094. eCollection 2019 Jul-Dec.
Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting cats worldwide. The objectives of the study were to estimate the prevalence of these retroviruses in domestic cats in Hungary and to characterise the phylogenetic relationships of FIV strains.
A total of 335 anticoagulated whole-blood samples obtained from both a healthy and ill cat population were examined for the presence of FIV and FeLV with two methods: ELISA and PCR. Statistical analysis was carried out to analyse the data obtained. Sequencing and phylogenetic analysis of partial polymerase () gene sequences was performed to describe circulating FIV subtypes.
Statistical analysis showed 11.8% and 9.9% true prevalence of FeLV and FIV, respectively, with ELISA. The apparent prevalence calculated from the PCR results were 17.3% for FeLV and 13.1% for FIV. Phylogenetic analysis of partial gene sequences obtained from 22 FIV strains showed that all observed Hungarian strains belonged to FIV subtype B. The strains were grouped into several monophyletic subgroups reflecting the geographic locations of the origin of the samples. The overall mean genetic similarity between the analysed strains was 98.2%.
We report the first thorough overview of the prevalence of FeLV and FIV in Hungary, which is relatively high, and give insight into the genetic diversity of Hungarian strains of FIV.
猫免疫缺陷病毒（FIV）和猫白血病病毒（FeLV）是影响全球猫的逆转录病毒。本研究的目的是估计这些逆转录病毒在匈牙利家猫中的流行率，并描述FIV毒株的系统发育关系。
采用酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）两种方法，对335份从健康和患病猫群体中采集的抗凝全血样本进行FIV和FeLV检测。对所得数据进行统计分析。对部分聚合酶（）基因序列进行测序和系统发育分析，以描述流行的FIV亚型。
ELISA统计分析显示，FeLV和FIV的实际流行率分别为11.8%和9.9%。根据PCR结果计算的表观流行率，FeLV为17.3%，FIV为13.1%。对22株FIV毒株的部分基因序列进行系统发育分析表明，所有观察到的匈牙利毒株均属于FIV B亚型。这些毒株被分为几个单系亚组，反映了样本来源的地理位置。分析毒株之间的总体平均遗传相似性为98.2%。
我们首次全面概述了匈牙利FeLV和FIV的流行情况，其流行率相对较高，并深入了解了匈牙利FIV毒株的遗传多样性。
