Department of Pharmaceutics, PSG College of Pharmacy, Coimbatore, India, 641 004.
Affiliated to The Tamilnadu Dr.M.G.R Medical University, Chennai, India.
Arch Microbiol. 2021 May;203(4):1707-1715. doi: 10.1007/s00203-020-02175-5. Epub 2021 Jan 18.
Staphylococcus aureus (S.aureus) is both a colonizer as well as a human pathogen that causes a variety of diseases. Mupirocin is a topical antimicrobial agent which is very effective against S.aureus infection. However, treating the S.aureus infection using mupirocin could be complicated due to biofilm formation. Consequently, resistance to mupirocin occurs and leads to chronic infection. The combination of mupirocin with a compound that has biofilm eradicating effect would be an ideal solution for effectively treating biofilm infections. Therefore, in this study, we have investigated the biofilm inhibitory and eradication effect of mupirocin with three essential oils (Cinnamon Oil (CO), Eugenol (EU) and Eucalyptus Oil (EO)) against sessile S.aureus. From these preliminary results, it was found that the mupirocin-CO (0.2 µg/ml-5.218 mg/ml) combination has a better synergistic antibiofilm effect against sessile S.aureus and the fractional inhibitory concentration index was found to be 0.458. The best combination of mupirocin with CO was loaded into a non-greasy O/W cream. The physico-chemical and microbiological evaluations were carried out for the prepared cream. The prepared cream has better biofilm eradication activity (40%) when compared to a marketed cream (20%).
金黄色葡萄球菌（S.aureus）既是定植菌也是人类病原体，可引起多种疾病。莫匹罗星是一种局部抗菌药物，对金黄色葡萄球菌感染非常有效。然而，由于生物膜的形成，使用莫匹罗星治疗金黄色葡萄球菌感染可能会变得复杂。因此，会产生对莫匹罗星的耐药性，从而导致慢性感染。将莫匹罗星与具有生物膜消除作用的化合物联合使用将是有效治疗生物膜感染的理想解决方案。因此，在这项研究中，我们研究了莫匹罗星与三种精油（肉桂油（CO）、丁香酚（EU）和桉树油（EO））联合使用对浮游金黄色葡萄球菌生物膜的抑制和消除作用。从这些初步结果发现，莫匹罗星-CO（0.2 µg/ml-5.218 mg/ml）组合对浮游金黄色葡萄球菌具有更好的协同抗生物膜作用，其部分抑菌浓度指数为 0.458。将莫匹罗星与 CO 的最佳组合载入无油腻性的 O/W 乳膏中。对制备的乳膏进行了物理化学和微生物学评价。与市售乳膏（20%）相比，制备的乳膏具有更好的生物膜消除活性（40%）。
