Kuang Yaming Honors School, Nanjing University, Nanjing, China.
Institute for Brain Sciences, Nanjing University, Nanjing, China.
Biopolymers. 2020 Oct;111(10):e23392. doi: 10.1002/bip.23392. Epub 2020 Sep 10.
Calcium release-activated calcium (CRAC) channels are highly calcium ion (Ca)-selective channels in the plasma membrane. The transient drop of endoplasmic reticulum Ca level activates its calcium sensor stromal interaction molecule (STIM) and then triggers the gating of the CRAC channel pore unit Orai. This process involves a variety of activities of the immune system. Therefore, understanding how the activation and regulation of the CRAC channel can be accomplished is essential. Here we briefly summarize the recent progress on Orai gating and its regulation by 2-aminoethoxydiphenylborate (2-APB) obtained from structural biology studies, biochemical and electrophysiological measurements, as well as molecular modeling. Indeed, integration between experiments and computations has further deepened our understanding of the channel gating and regulation.
钙释放激活钙 (CRAC) 通道是质膜中高度钙离子 (Ca) 选择性通道。内质网 Ca 水平的短暂下降会激活其钙传感器基质相互作用分子 (STIM)，然后触发 CRAC 通道孔单位 Orai 的门控。这个过程涉及免疫系统的各种活动。因此，了解如何激活和调节 CRAC 通道是至关重要的。在这里，我们简要总结了近年来从结构生物学研究、生化和电生理测量以及分子建模中获得的关于 Orai 门控及其被 2-氨基乙氧基二苯硼酸盐 (2-APB) 调节的最新进展。事实上，实验和计算的整合进一步加深了我们对通道门控和调节的理解。
