Department of Pathology, Zibo Central Hospital, Zibo 255000, Shandong Province, China.
Department of Pathology, Zibo Fourth People's Hospital, Zibo 255067, Shandong Province, China.
Aging (Albany NY). 2021 Jan 13;13(2):2941-2958. doi: 10.18632/aging.202444.
Gallbladder carcinoma (GC) is an extremely malignant gastrointestinal tumor, but relevant mechanisms are still under investigation. MicroRNA (miR) is differentially expressed in a variety of tumors. Here we explored miR-204 in patients with GC and related mechanisms. A GSE104165 chip was downloaded from the gene expression omnibus (GEO) for analysis. The qRT-PCR assay was used for quantifying miR-204 and Notch2 in the serum and tissues of the patients, and the patients were followed up for 3 years to analyze independent factors of prognosis. The CCK8, transwell, and flow cytometry assays were applied for analyzing proliferation, invasion, as well as apoptosis of cells, and the dual luciferase reporter (DLR) assay was adopted for determining the association of miR-204 with Notch2. MiR-204 was low in patients with GC, and it might serve as a diagnostic indicator for GC. In addition, patients with low e MiR-204 usually faced high rates of III+IV stage, distant metastasis, and low differentiation, and also showed a poor prognosis. DLR assay verified the targeted binding of miR-204 to Notch2 mRNA.
胆囊癌（GC）是一种极其恶性的胃肠道肿瘤，但相关机制仍在研究中。MicroRNA（miR）在多种肿瘤中表达差异。在这里，我们研究了 GC 患者的 miR-204 及其相关机制。从基因表达综合数据库（GEO）下载了 GSE104165 芯片进行分析。采用 qRT-PCR 法检测患者血清和组织中 miR-204 和 Notch2 的表达水平，并对患者进行 3 年随访，分析预后的独立因素。采用 CCK8、Transwell 和流式细胞术检测细胞增殖、侵袭和凋亡，采用双荧光素酶报告（DLR）检测 miR-204 与 Notch2 的相关性。GC 患者 miR-204 水平较低，可能作为 GC 的诊断指标。此外，miR-204 低表达的患者通常处于 III+IV 期、远处转移和低分化，预后不良。DLR 检测证实 miR-204 与 Notch2 mRNA 存在靶向结合。
