在长双歧杆菌亚种婴儿 ATCC 15697 中生成岩藻糖通透酶缺失突变体。
Generating a fucose permease deletion mutant in Bifidobacterium longum subspecies infantis ATCC 15697.
机构信息
Department of Chemistry, The University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.
Department of Chemistry, The University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.
出版信息
Anaerobe. 2021 Apr;68:102320. doi: 10.1016/j.anaerobe.2021.102320. Epub 2021 Jan 16.
Bifidobacterium longum subsp. infantis ATCC 15697 has emerged as a model for infant gut-associated bifidobacterial strains. Here we present a genetic system for B. longum subsp. infantis ATCC 15697 using its own DNA restriction-modification systems and create a fucose permease deletion mutant lacking the ability to use free fucose as a carbon source.
长双歧杆菌亚种。婴儿 ATCC 15697 已成为婴儿肠道相关双歧杆菌菌株的模型。在这里，我们使用其自身的 DNA 限制修饰系统为长双歧杆菌亚种。婴儿 ATCC 15697 展示了一个遗传系统，并创建了一个缺乏使用游离岩藻糖作为碳源能力的岩藻糖通透酶缺失突变体。
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