Institute of Physiology, Department of Neurophysiology, Eberhard Karls University of Tübingen, Tübingen, Germany.
Institute of Physiology, Department of Neurophysiology, Eberhard Karls University of Tübingen, Tübingen, Germany.
Cell Calcium. 2021 Mar;94:102334. doi: 10.1016/j.ceca.2020.102334. Epub 2021 Jan 11.
S100B is an EF-hand type Ca-binding protein of the S100 family, known to support neurogenesis and to promote the interactions between brain's nervous and immune systems. Here, we characterized the expression of S100B in the mouse olfactory bulb, a neurogenic niche comprising mature and adult-born neurons, astrocytes, oligodendrocytes and microglia. Besides astrocytes, for which S100B is a classical marker, S100B was also expressed in NG2 cells and, surprisingly, in APC-positive myelinating oligodendrocytes but not in mature/adult-born neurons or microglia. Various layers of the bulb differed substantially in the composition of S100B-positive cells, with the highest fraction of the APC-positive oligodendrocytes found in the granule cell layer. Across all layers, ∼50 % of NG2 cells were S100B-negative. Finally, our data revealed a strong correlation between the fraction of myelinating oligodendrocytes among the S100B-positive cells and the oligodendrocyte density in different brain areas, underscoring the importance of S100B for the establishment and maintenance of myelin sheaths.
S100B 是 S100 家族中的一种 EF 手型钙结合蛋白，已知它能支持神经发生，并促进大脑神经系统和免疫系统之间的相互作用。在这里，我们描述了 S100B 在小鼠嗅球中的表达情况，嗅球是一个神经发生龛，包含成熟和成年新生神经元、星形胶质细胞、少突胶质细胞和小胶质细胞。除了星形胶质细胞，S100B 是其经典标志物，S100B 也在 NG2 细胞中表达，令人惊讶的是，在 APC 阳性的髓鞘形成少突胶质细胞中表达，但不在成熟/成年新生神经元或小胶质细胞中表达。嗅球的各个层在 S100B 阳性细胞的组成上有很大差异，在颗粒细胞层中发现 APC 阳性的少突胶质细胞比例最高。在所有层中，约 50%的 NG2 细胞 S100B 阴性。最后，我们的数据显示，S100B 阳性细胞中髓鞘形成少突胶质细胞的比例与不同脑区的少突胶质细胞密度之间存在很强的相关性，这突显了 S100B 对髓鞘形成和维持的重要性。
