Department of Chemistry and Industrial Chemistry, University of Pisa, Via G. Moruzzi 13, 56124 Pisa, Italy.
Institute of Life Sciences, Sant'Anna School of Advanced Study, Piazza Martiri della Libertà 33, 56127 Pisa, Italy.
Bioresour Technol. 2021 Apr;325:124635. doi: 10.1016/j.biortech.2020.124635. Epub 2020 Dec 30.
Giant reed (Arundo donax L.) is a promising source of carbohydrates that can be converted into single cell oil (SCO) by oleaginous yeasts. Microbial conversion of both hemicellulose and cellulose fractions represents the key step for increasing the economic sustainability for SCO production. Lipomyces starkeyi DSM 70,296 was cultivated in two xylose-rich hydrolysates, obtained by the microwave-assisted hydrolysis of hemicellulose catalysed by FeCl or Amberlyst-70, and in two glucose-rich hydrolysates obtained by the enzymatic hydrolysis of cellulose. L. starkeyi grew on both undetoxified and partially-detoxified hydrolysates, achieving the lipid content of 30 wt% and yield values in the range 15-24 wt%. For both integrated cascade processes the final production of about 8 g SCO from 100 g biomass was achieved. SCO production through integrated hydrolysis cascade processes represents a promising solution for the effective exploitation of lignocellulosic feedstock from perennial grasses towards new generation biodiesel and other valuable bio-based products.
芦竹（Arundo donax L.）是一种很有前途的碳水化合物来源，可以通过产油酵母转化为单细胞油（SCO）。通过油脂酵母将半纤维素和纤维素部分转化，是提高 SCO 生产经济可持续性的关键步骤。在两种富含木糖的水解物中培养了嗜木聚糖脂酵母（Lipomyces starkeyi）DSM 70,296，这两种水解物是通过 FeCl 或 Amberlyst-70 催化的微波辅助半纤维素水解获得的，以及两种由纤维素酶解获得的富含葡萄糖的水解物。L. starkeyi 在未经解毒和部分解毒的水解物上均能生长，达到了 30wt%的脂质含量和 15-24wt%的产率值。对于这两种集成级联工艺，最终从 100g 生物质中生产了约 8g SCO。通过集成水解级联工艺生产 SCO 是一种很有前途的解决方案，可有效利用多年生草本植物的木质纤维素原料，生产新一代生物柴油和其他有价值的生物基产品。
