Beijing Scientific Observation and Experiment Station for Veterinary Drugs and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, China /Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China.
BMC Vet Res. 2021 Jan 18;17(1):36. doi: 10.1186/s12917-020-02740-6.
Vesicular stomatitis (VS) is an acute, highly contagious and economically important zoonotic disease caused by the vesicular stomatitis virus (VSV). There is a need for effective and safe stable recombinant vaccine for the control of the disease. The human type 5 replication-defective adenovirus expression vector is a good way to construct recombinant vaccines.
Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity. G proteins were successfully expressed with good immunogenicity. The rAds could induce the production of VSV antibodies in mice, and VSV neutralizing antibodies in goats, respectively. The neutralizing antibody titers could reach 1:32 in mice and 1:64 in goats. The rAds induced strong lymphocyte proliferation in mice and goats, which was significantly higher compared to the negative control groups.
The three rAds constructed in the study expressed VSV-G proteins and induced both humoral and cellular immune responses in mice and goats. These results lay the foundation for further studies on the use of rAds in vaccines expressing VSV-G.
水疱性口炎（VS）是一种由水疱性口炎病毒（VSV）引起的急性、高度传染性和具有重要经济意义的人畜共患病。需要有效的、安全的稳定重组疫苗来控制该疾病。人 5 型复制缺陷型腺病毒表达载体是构建重组疫苗的一种好方法。
成功构建了三种重组腺病毒（rAd），它们分别表达了 VSV 印第安纳血清型糖蛋白（VSV-IN-G）、VSV 新泽西血清型糖蛋白（VSV-NJ-G）和 G 融合蛋白（两种血清型 G [VSV-IN-G-NJ-G]），具有诱导保护性免疫的潜力。G 蛋白成功表达，具有良好的免疫原性。rAd 能够在小鼠中诱导产生 VSV 抗体，在山羊中诱导产生 VSV 中和抗体。中和抗体滴度在小鼠中可达 1:32，在山羊中可达 1:64。rAd 在小鼠和山羊中诱导强烈的淋巴细胞增殖，与阴性对照组相比显著升高。
本研究构建的三种 rAd 表达了 VSV-G 蛋白，并在小鼠和山羊中诱导了体液和细胞免疫反应。这些结果为进一步研究 rAd 在表达 VSV-G 的疫苗中的应用奠定了基础。
