Department of Allergy and Clinical Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Virology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Allergy Asthma Immunol. 2020 Oct 18;19(5):471-477. doi: 10.18502/ijaai.v19i5.4462.
The emergence of a highly pathogenic virus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) accounts for severe pneumonia throughout the world. More than 7 million world population have been infected with SARS-CoV-2, and the number of deaths is increasing every day. This study aimed to evaluate the frequency of SARS-CoV-2 in hospitalized patients with an acute respiratory infection (ARI). During an outbreak of the SARS-CoV-2, the nasopharyngeal and oropharyngeal swabs were collected from 909 hospitalized patients with severe pneumonia, including 517 (56.9%) males and 392 (43.1%) females. All the collected samples were from different cities of Khuzestan province from 19 February to- 27 March 2020. The RNA was extracted from samples and subjected to real-time polymerase chain reaction (PCR) tests for the detection of the SARS-CoV-2. Simultaneously, the computerized tomography (CT) scan was tested for the presence of ground-glass opacity in the lung among the patients. Of the total number of 909 specimens, 328 (36.08%) cases, including 185 (20.35%) females and 143 (15.73%) males, were positive for the SARS-CoV-2 while, 581 (63.9%) cases, including 374 (41.14%) males and 207 (22.77%) were negative for the SARS-CoV-2 by real-time PCR (p=0.001).Four hundred sixteen (45.76%) cases were positive for ground-glass opacity in the lung by CT scan, while 328/909 (36.08%) trials proved positive for SARS-CoV-2 by the real-time PCR (p=0.003). In this study, 36.08% of patients were positive for SARS-CoV-2. Although the results of positive cases by CT scan showed higher than real-time PCR, screening the SARS-COV-2 with a real-time PCR method is the first line of choice.
一种名为严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的高致病性病毒的出现导致了全球范围内的严重肺炎。超过 700 万世界人口感染了 SARS-CoV-2，并且死亡人数每天都在增加。本研究旨在评估住院急性呼吸道感染（ARI）患者中 SARS-CoV-2 的频率。在 SARS-CoV-2 爆发期间，从来自不同城市的 909 名重症肺炎住院患者中采集了鼻咽和口咽拭子，其中 517 名（56.9%）为男性，392 名（43.1%）为女性。所有采集的样本均来自胡齐斯坦省，采集时间为 2020 年 2 月 19 日至 3 月 27 日。从样本中提取 RNA，并进行实时聚合酶链反应（PCR）检测以检测 SARS-CoV-2。同时，对患者肺部的磨玻璃样混浊进行计算机断层扫描（CT）检查。在总共 909 个标本中，有 328 例（36.08%），包括 185 例（20.35%）女性和 143 例（15.73%）男性，SARS-CoV-2 检测呈阳性，而 581 例（63.9%），包括 374 例（41.14%）男性和 207 例（22.77%）男性，实时 PCR 检测 SARS-CoV-2 阴性（p=0.001）。416 例（45.76%）患者的 CT 扫描显示肺部有磨玻璃样混浊，而 328/909 例（36.08%）试验通过实时 PCR 检测 SARS-CoV-2 呈阳性（p=0.003）。在本研究中，有 36.08%的患者 SARS-CoV-2 检测呈阳性。尽管 CT 扫描阳性结果显示高于实时 PCR，但使用实时 PCR 方法筛查 SARS-COV-2 是首选方法。
