Wang Yuqing, Yao Danyu, Li Linhao, Qian Zhiyong, He Wei, Ding Rui, Liu Haifeng, Fan Yubo
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People's Republic of China.
School of Automation Hangzhou Dianzi University, Hangzhou 310018, People's Republic of China.
ACS Biomater Sci Eng. 2020 Jun 8;6(6):3502-3512. doi: 10.1021/acsbiomaterials.0c00175. Epub 2020 May 20.
Biomaterial implantation is followed by an inflammatory cascade dominated by the macrophages, which polarized to the proinflammation M1 phenotype or prohealing M2 phenotype. Generally, silk sericin (SS) is considered to be of high immunogenicity associated with native silk fibers. The blends of silk fibroin (SF) and SS in different mass ratios might elicit different host immune responses and induce macrophage phenotype switch. The objective of this study was to assess the effects of electrospun SF-SS fibrous films with different mass ratios (10:0, 9:1, 8:2, and 7:3) on the macrophage phenotypes and explore the optimal ratio of SF and SS for angiogenesis. Our results indicated that the macrophages were activated by the addition of SS. When the mass ratio of SF and SS reached 7:3, the film displayed the highest degree of vascularization. The macrophages were induced to secrete more M1 and M2 cytokines accompanying with high M2/M1 ratio. Taken together, this study provided a perspective to promote neovascularization by modulating appropriate host response and macrophage phenotypes in tissue engineering field.
生物材料植入后会引发以巨噬细胞为主导的炎症级联反应，巨噬细胞会极化为促炎M1表型或促愈合M2表型。一般来说，丝胶蛋白（SS）被认为与天然丝纤维相关，具有高免疫原性。不同质量比的丝素蛋白（SF）和SS混合物可能会引发不同的宿主免疫反应并诱导巨噬细胞表型转换。本研究的目的是评估不同质量比（10:0、9:1、8:2和7:3）的静电纺丝SF-SS纤维膜对巨噬细胞表型的影响，并探索促进血管生成的SF和SS的最佳比例。我们的结果表明，添加SS可激活巨噬细胞。当SF与SS的质量比达到7:3时，该膜显示出最高程度的血管化。巨噬细胞被诱导分泌更多的M1和M2细胞因子，且M2/M1比值较高。综上所述，本研究为在组织工程领域通过调节适当的宿主反应和巨噬细胞表型来促进新血管形成提供了一个视角。
