Panilaitis Bruce, Altman Gregory H, Chen Jingsong, Jin Hyoung Joon, Karageorgiou Vassilis, Kaplan David L
Department of Biomedical Engineering, Tufts University, Bioengineering Center, 4 Colby Street, Medford, Massachusetts, MA 02155, USA.
Biomaterials. 2003 Aug;24(18):3079-85. doi: 10.1016/s0142-9612(03)00158-3.
Silk fibers have potential biomedical applications beyond their traditional use as sutures. The physical properties of silk fibers and films make it a promising candidate for tissue engineering scaffold applications, particularly where high mechanical loads or tensile forces are applied or in cases where low rates of degradation are desirable. A critical issue for biomaterial scaffolds is biocompatibility. The direct inflammatory potential of intact silk fibers as well as extracts was studied in an in vitro system. The results indicate that silk fibers are largely immunologically inert in short- and long-term culture with RAW 264.7 murine macrophage cells while insoluble fibroin particles induced significant TNF release. Soluble sericin proteins extracted from native silk fibers did not induce significant macrophage activation. While sericin did not activate macrophages by itself, it demonstrated a synergistic effect with bacterial lipopolysaccharide. The low level of inflammatory potential of silk fibers makes them promising candidates in future biomedical applications.
丝绸纤维除了传统上用作缝合线外，还具有潜在的生物医学应用。丝绸纤维和薄膜的物理特性使其成为组织工程支架应用的有前途的候选材料，特别是在施加高机械负荷或拉力的情况下，或者在需要低降解率的情况下。生物材料支架的一个关键问题是生物相容性。在体外系统中研究了完整丝绸纤维及其提取物的直接炎症潜力。结果表明，在与RAW 264.7小鼠巨噬细胞的短期和长期培养中，丝绸纤维在很大程度上是免疫惰性的，而不溶性丝素蛋白颗粒会诱导大量肿瘤坏死因子（TNF）释放。从天然丝绸纤维中提取的可溶性丝胶蛋白不会诱导显著的巨噬细胞激活。虽然丝胶蛋白本身不会激活巨噬细胞，但它与细菌脂多糖表现出协同作用。丝绸纤维的低炎症潜力使其成为未来生物医学应用中有前途的候选材料。
