Duran G Ler Seda, Balbaba Mehmet, Çolakoğlu Neriman, Bulmuş Özgör, Ulaş Fatih, Eröksüz Yesari
Department of Ophthalmology, Faculty of Medicine, Fırat University, Elazığ, Turkey.
Department of Histology-Embryology, Faculty of Medicine, Fırat University, Elazığ, Turkey.
Indian J Ophthalmol. 2021 Feb;69(2):369-373. doi: 10.4103/ijo.IJO_1739_20.
The aims of this study were to evaluate the effects of decorin (DCN) in rat oxygen-induced retinopathy (OIR) model and to compare the results with those of bevacizumab.
Twenty-eight newborn Sprague-Dawley rats were randomly divided into four groups. Group I (control): normoxia plus intraperitoneal (ip) normal saline (NS), Group II (sham): OIR plus ip NS, Group III (DCN): OIR plus ip 0.1 mg/kg DCN, and Group IV (bevacizumab): OIR plus ip 2.5 mg/kg bevacizumab. The OIR model was induced by cycling the oxygen concentration between 50% and 10% every 24 h for 14 days following their birth. In all groups, injections were administered on postnatal day (PD) 15. All animals were sacrificed and their right eyes were enucleated on PD 18. The nuclei of neovascular endothelial cells on the vitreal side of the inner limiting membrane were counted, and vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF)-α immunoreactivity were detected in histopathological and immunohistochemical examinations. One-way analysis of variance and post hoc Tukey tests were used for statistical analyses of the data.
In Groups II, III, and IV, the mean neovascular cell nuclei counts were 13.14 ± 1.34, 6.57 ± 1.51, and 6.71 ± 1.49, respectively. The mean neovascular cell nuclei count was significantly reduced in treatment groups compared with sham group (P < 0.001). In immunohistochemical staining, the immunoreactivity of VEGF was 0.07 ± 0.02, 0.97 ± 0.21, 0.37 ± 0.12, and 0.23 ± 0.17, respectively. Likewise, immunoreactivity of TNF-α was 0.02 ± 0.02, 1.11 ± 0.36, 0.37 ± 0.13, and 0.62 ± 0.21, respectively. VEGF and TNF-α immunoreactivity increased markedly in the sham group compared with those in the control group (P < 0.001). VEGF and TNF-α immunoreactivity of treatment groups decreased significantly compared to sham group (P < 0.001).
The beneficial effects obtained by DCN administration in OIR model were comparable to the effects of bevacizumab.
本研究旨在评估核心蛋白聚糖（DCN）在大鼠氧诱导视网膜病变（OIR）模型中的作用，并将结果与贝伐单抗的结果进行比较。
28只新生Sprague-Dawley大鼠随机分为四组。第一组（对照组）：常氧加腹腔注射（ip）生理盐水（NS）；第二组（假手术组）：OIR加ip NS；第三组（DCN组）：OIR加ip 0.1 mg/kg DCN；第四组（贝伐单抗组）：OIR加ip 2.5 mg/kg贝伐单抗。出生后每24小时将氧浓度在50%和10%之间循环14天以诱导OIR模型。所有组均在出生后第15天（PD15）进行注射。所有动物在PD18处死并摘除右眼。对内界膜玻璃体侧的新生血管内皮细胞核进行计数，并在组织病理学和免疫组织化学检查中检测血管内皮生长因子（VEGF）和肿瘤坏死因子-α（TNF）-α免疫反应性。数据的统计分析采用单因素方差分析和事后Tukey检验。
在第二、三、四组中，新生血管细胞核的平均计数分别为13.14±1.34、6.57±1.51和6.71±1.49。与假手术组相比，治疗组新生血管细胞核的平均计数显著降低（P<0.001）。在免疫组织化学染色中，VEGF的免疫反应性分别为0.07±0.02、0.97±0.21、0.37±0.12和0.
