Department of Ophthalmology, Faculty of Medicine, Bolu Abant Izzet Baysal University, Bolu, Turkey.
Department of Physiology, Faculty of Medicine, Bolu Abant Izzet Baysal University, Bolu, Turkey.
Indian J Ophthalmol. 2021 May;69(5):1219-1223. doi: 10.4103/ijo.IJO_1935_20.
Investigating the effects of intraperitoneal carvacrol administration in rats using the oxygen-induced retinopathy (OIR) model.
A total of 28 newborn Sprague Dawley rats were used and the OIR model was created using the 50/10% oxygen model. The study composed of four groups in total. While the OIR model was not used in Group I (control group), it was created for Groups II, III, and IV. About 0.01 mL carvacrol, bevacizumab, or 0.9% NaCl was administered intraperitoneal (IP) to the rats in all groups on postnatal day (PND) 14 as follows: Group I and Group II were administered 0.9% NaCl, Group III was administered bevacizumab, and Group IV was administered carvacrol. On PND 18, rats were sacrificed and their right eyes were enucleated.
Histopathological and immunohistochemical studies showed that the number of vascular endothelial cells (VECs), vascular endothelial growth factor (VEGF), and tumor necrosis factor-α (TNF-α) decreased similarly in Group III and Group IV compared with Group II. VECs values for Group I, Group II, Group III, and Group IV were measured as 0 ± 0, 26.45 ± 4.57, 7.75 ± 1.98, and 5.78 ± 1.72, respectively, and it differed significantly between groups (P < 0.001). Likewise, VEGF levels were observed as 0.06 ± 0.01, 3.31 ± 0.53, 2.47 ± 0.44, and 2.49 ± 0.52, respectively, and it differed significantly between groups (P < 0.001). TNF-α levels were recorded as 0.06 ± 0.01, 3.58 ± 0.38, 2.46 ± 0.49, and 2.29 ± 0.25, respectively, and it differed significantly between groups (P < 0.001). VECs, VEGF, and TNF-α were similar between Group III and IV (range of P values were 0.486-0.998).
The study demonstrated that carvacrol significantly reduced retinal pathological angiogenesis, NV, VEC nuclei count, VEGF, and TNF-α levels. Moreover, the observed effects were comparable to those of bevacizumab.
通过氧诱导视网膜病变（OIR）模型研究腹腔内给予香芹酚对大鼠的影响。
共使用 28 只新生 Sprague Dawley 大鼠，使用 50/10%氧模型建立 OIR 模型。研究共分为四组。第 I 组（对照组）未使用 OIR 模型，第 II 组、第 III 组和第 IV 组建立了 OIR 模型。在生后第 14 天，各组大鼠腹腔内分别给予 0.01 mL 香芹酚、贝伐单抗或 0.9%生理盐水：第 I 组和第 II 组给予 0.9%生理盐水，第 III 组给予贝伐单抗，第 IV 组给予香芹酚。在生后第 18 天处死大鼠，取出右眼。
组织病理学和免疫组织化学研究表明，与第 II 组相比，第 III 组和第 IV 组的血管内皮细胞（VEC）、血管内皮生长因子（VEGF）和肿瘤坏死因子-α（TNF-α）数量减少相似。第 I 组、第 II 组、第 III 组和第 IV 组的 VEC 值分别为 0±0、26.45±4.57、7.75±1.98 和 5.78±1.72，组间差异有统计学意义（P<0.001）。同样，VEGF 水平分别为 0.06±0.01、3.31±0.53、2.47±0.44 和 2.49±0.52，组间差异有统计学意义（P<0.001）。TNF-α 水平分别为 0.06±0.01、3.58±0.38、2.46±0.49 和 2.29±0.25，组间差异有统计学意义（P<0.001）。第 III 组和第 IV 组的 VEC、VEGF 和 TNF-α 相似（范围 P 值为 0.486-0.998）。
本研究表明香芹酚可显著降低视网膜病理性血管生成、NV、VEC 核计数、VEGF 和 TNF-α 水平。此外，观察到的效果与贝伐单抗相当。
