Department of Chemical Sciences, University of Napoli "Federico II," Via Cintia, Napoli, Italy.
Istituto di Biostrutture e Bioimmagini, CNR, Napoli, Italy.
Biotechnol Appl Biochem. 2020 Jul;67(4):549-562. doi: 10.1002/bab.1969. Epub 2020 Jun 26.
Artificial metalloenzymes as catalysts are promising candidates for their use in different technologies, such as bioremediation, biomass transformation, or biosensing. Despite this, their practical exploitation is still at an early stage. Immobilized natural enzymes have been proposed to enhance their applicability. Immobilization may offer several advantages: (i) catalyst reuse; (ii) easy separation of the enzyme from the reaction medium; (iii) better tolerance to harsh temperature and pH conditions. Here, we report an easy immobilization procedure of an artificial peroxidase on different surfaces, by means of click chemistry. FeMC6a, a recently developed peroxidase mimic, has been functionalized with a pegylated aza-dibenzocyclooctyne to afford a "clickable" biocatalyst, namely FeMC6a-PEG@DBCO, which easily reacts with azide-functionalized molecules and/or nanomaterials to afford functional bioconjugates. The clicked biocatalyst retains its structural and, to some extent, its functional behaviors, thus housing high potential for biotechnological applications.
人工金属酶作为催化剂在生物修复、生物质转化或生物传感等不同技术中有很大的应用潜力。尽管如此，它们的实际应用仍处于早期阶段。固定化天然酶已被提议用于提高其适用性。固定化可能具有以下优点：（i）催化剂可重复使用；（ii）酶与反应介质易于分离；（iii）对恶劣温度和 pH 条件的耐受性更好。在这里，我们报告了一种通过点击化学将人工过氧化物酶固定在不同表面上的简便方法。FeMC6a 是一种最近开发的过氧化物酶模拟物，用聚乙二醇化的氮杂二苯并环辛炔官能化，得到一种“点击式”生物催化剂，即 FeMC6a-PEG@DBCO，它很容易与叠氮化物官能化的分子和/或纳米材料反应，得到功能化的生物缀合物。点击化的生物催化剂保留了其结构，在某种程度上保留了其功能行为，因此具有很高的生物技术应用潜力。
