一种用于测量未提取血浆中VP16（依托泊苷）的酶联免疫吸附测定法。

An ELISA for the measurement of VP16 (etoposide) in unextracted plasma.

作者信息

Henneberry H P, Aherne G W, Marks V

机构信息

Department of Biochemistry, University of Surrey, Guildford, U.K.

出版信息

J Immunol Methods. 1988 Mar 16;107(2):205-9. doi: 10.1016/0022-1759(88)90219-0.

DOI:10.1016/0022-1759(88)90219-0

PMID:3346552

Abstract

An ELISA for the anti-cancer drug VP16 in unextracted plasma has been developed using VP16-thyroglobulin-coated microtitre plates, a sheep anti-VP16 serum, and a donkey anti-sheep HRPO-labelled second antibody. The sensitivity of the assay was 0.5 ng/ml, providing a detection limit of 0.1 microgram VP16/ml plasma. Plasma interference effects were negligible and therefore the standard curve could be set up in assay buffer. A good correlation was obtained between the ELISA and established HPLC and RIA methods. No evidence was found of significant levels of cross-reacting metabolites in plasma samples obtained from patients receiving VP16 therapy. The antiserum did not cross-react with the epiaglycone of VP16.

摘要

已开发出一种用于检测未提取血浆中抗癌药物VP16的酶联免疫吸附测定法（ELISA），该方法使用包被有VP16-甲状腺球蛋白的微量滴定板、羊抗VP16血清以及驴抗羊辣根过氧化物酶标记的二抗。该测定法的灵敏度为0.5纳克/毫升，血浆中VP16的检测限为0.1微克/毫升。血浆干扰效应可忽略不计，因此可在测定缓冲液中建立标准曲线。ELISA法与既定的高效液相色谱法（HPLC）和放射免疫分析法（RIA）之间具有良好的相关性。在接受VP16治疗的患者的血浆样本中，未发现有显著水平的交叉反应性代谢物。抗血清与VP16的表阿糖苷配基无交叉反应。