从一名61岁中国汉族健康女性供体生成人诱导多能干细胞系（SIAISi011-A）。
Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor.
作者信息
Zhang Wenxin, Hua Yishi, Di Weihao, Guo Jinghui, Dai Qiuting, Zhao Jian, Zhang Bei, Wang Ying
机构信息
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.
出版信息
Stem Cell Res. 2021 Mar;51:102173. doi: 10.1016/j.scr.2021.102173. Epub 2021 Jan 12.
Peripheral blood mononuclear cells (PBMC) were donated by a healthy 61-year old Chinese Han female. Human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors were used to reprogram her PBMCs with the non-integrating episomal vector system. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs and their ability to differentiate spontaneously three germ layers in vitro. The iPSC line displayed a normal karyotype. Our model offers the possibility to be used a control in pathological mechanism studies.
外周血单个核细胞（PBMC）由一名61岁健康中国汉族女性捐赠。使用人类OKSM（OCT3/4、KLF4、SOX2和c-MYC）转录因子，通过非整合型附加体质载体系统对其PBMC进行重编程。多能性标志物的免疫细胞化学证实了无转基因诱导多能干细胞（iPSC）的多能性及其在体外自发分化为三个胚层的能力。该iPSC系显示出正常的核型。我们的模型为病理机制研究提供了用作对照的可能性。
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