Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China.
Stem Cell Res. 2021 May;53:102314. doi: 10.1016/j.scr.2021.102314. Epub 2021 Mar 26.
A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies.
一位健康的 31 岁中国汉族女性捐献了外周血单个核细胞（PBMC）。她的 PBMC 被非整合型附加体载体系统重编程为人类 OKSM（OCT3/4、KLF4、SOX2 和 c-MYC）转录因子。多能性标志物的免疫细胞化学染色证实了无转基因 iPSCs 的多能性。还证实了它们在体外自发分化为三个胚层的能力。iPSC 系显示正常核型。该模型可用于病理机制研究的对照。
