TUSMi003-A的建立，这是一株来自一名62岁具有ApoE3/4基因背景的中国汉族阿尔茨海默病患者的诱导多能干细胞（iPSC）系。
Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background.
作者信息
Wang Ying, Yu Hongxiang, Zhang Yue, Zhang Jing, Chen Keni, Sun Heng, Meng Shulin, Li Gang, Lei Ying, Zhao Jian
机构信息
Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
出版信息
Stem Cell Res. 2018 Mar;27:57-60. doi: 10.1016/j.scr.2018.01.005. Epub 2018 Jan 4.
A 62-year old Chinese Han Alzheimer's disease (AD) female patient with ApoE3/4 genetic background donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogrammed PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.
一名62岁具有ApoE3/4基因背景的中国汉族阿尔茨海默病（AD）女性患者捐赠了她的外周血单个核细胞（PBMC）。使用非整合附加型载体系统，用人OKSM转录因子对PBMC进行重编程。通过免疫细胞化学检测多能性标志物以及iPSC在体外自发分化为三个胚层的能力，证实了无转基因iPSC的多能性。此外，该iPSC系显示出正常的核型。我们的模型可能为进一步研究病理机制、鉴定早期生物标志物以及进行AD药物测试研究提供一个良好的平台。
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